Pintér C, Siccardi A G, Clivio A
Dipartimento di Biologia e Genetica per le Scienze Mediche, Università degli Studi di Milano, Italy.
Cell Biol Int. 1995 Jun;19(6):507-15. doi: 10.1006/cbir.1995.1095.
A human T cell line chronically infected with Human Immunodeficiency Virus (HIV) has been adapted to grow in a chemically defined, protein-free medium. Virus particles are produced at rates comparable to those of serum-supplemented cultures; virus preparations free of undesirable proteins can be produced in preparative amounts by simple ultrafiltration procedures and cell culture supernatants can be used as such for the preparation of ELISA solid phases. This material has been used very conveniently for studies concerning characterization of antibodies against HIV-specific proteins, interaction of HIV with complement components and inclusion of human cell-derived proteins into virions; we propose its use as a powerful tool for the structural as well as functional analysis of the virus particle itself.
一种长期感染人类免疫缺陷病毒（HIV）的人T细胞系已被改造，使其能在化学成分明确的无蛋白培养基中生长。病毒颗粒的产生速率与补充血清的培养物相当；通过简单的超滤程序可以制备出不含不良蛋白质的病毒制剂，且细胞培养上清液可直接用于制备ELISA固相。这种材料非常便于用于研究抗HIV特异性蛋白的抗体特性、HIV与补体成分的相互作用以及人类细胞衍生蛋白纳入病毒粒子的情况；我们建议将其用作对病毒粒子本身进行结构和功能分析的有力工具。
