von Eggeling F, Spielvogel H
Institut für Humangenetik und Anthropologie, Klinikum der FSU Jena, Germany.
Cell Mol Biol (Noisy-le-grand). 1995 Jul;41(5):653-70.
Since the technique of PCR was developed in 1985 (Saiki et al). many new forms of applications have been described in the literature. Of particular interest are PCR approaches, allowing amplification of unknown sequences. In this review these approaches are generally termed "Random PCR"s. While "conventional PCR" in the form that was first described by Saiki et al. is utilized for the amplification and subsequent detection of specific DNA sequences, which are precisely characterized in length and sequence, Random PCR is either used for universal amplification of prevailing DNA or for amplification of unknown intervening sequences which are not generally defined in length or sequence. We depict criteria for discrimination between conventional PCR and Random PCR. Furthermore, we have compiled a classification system for Random PCR approaches based on differentiation by primer structure (degenerate--non-degenerate). According to this classification system a general overview of published Random PCR approaches is given. Future aspects of application of Random PCR are mentioned and commented. Own investigations, combining Random PCR with the technique of chromosome microdissection and thus allowing characterization of unknown chromosomes or chromosome fragments via FISH, are presented. Results are briefly summarized.
自1985年PCR技术问世以来（萨奇等人），文献中描述了许多新的应用形式。特别值得关注的是PCR方法，它能够扩增未知序列。在本综述中，这些方法通常被称为“随机PCR”。虽然萨奇等人首次描述的“传统PCR”用于扩增并随后检测长度和序列均已精确表征的特定DNA序列，但随机PCR要么用于普遍扩增占主导地位的DNA，要么用于扩增长度或序列通常未明确界定的未知间隔序列。我们描述了区分传统PCR和随机PCR的标准。此外，我们基于引物结构（简并型——非简并型）的差异，编制了一个随机PCR方法的分类系统。根据这个分类系统，对已发表的随机PCR方法进行了总体概述。文中提到并评论了随机PCR未来的应用前景。展示了我们自己的研究，即将随机PCR与染色体显微切割技术相结合，从而通过荧光原位杂交（FISH）对未知染色体或染色体片段进行表征。对结果进行了简要总结。
