用于检测导致牛白细胞黏附缺陷的突变的聚合酶链反应（PCR）检测方法的优化。
Optimization of the PCR test for the mutation causing bovine leukocyte adhesion deficiency.
作者信息
Mirck M H, Von Bannisseht-Wijsmuller T, Timmermans-Besselink W J, Van Luijk J H, Buntjer J B, Lenstra J A
机构信息
Animal Health Service, Deventer, The Netherlands.
出版信息
Cell Mol Biol (Noisy-le-grand). 1995 Jul;41(5):695-8.
The recent emergence of the bovine leukocyte adhesion deficiency (BLAD) demonstrated the risks of narrowing the genetic basis of a population. About 6% of the Holstein-Friesian cattle now descends from one bull who was a heterozygous BLAD carrier. Crossing his descendants resulted in the birth of homozygous BLAD calves with a life expectancy of < 1 year. The BLAD syndrome is caused by a point mutation in the gene coding for CD18, a subunit of the beta 2 integrins on the surface of leukocytes. By using a PCR-RFLP test, large numbers of cattle are now being screened in several countries to eradicate the mutant allele. We describe an optimization of the PCR primer set that has led to an improvement of the test.
牛白细胞黏附缺陷症（BLAD）最近的出现证明了缩小种群遗传基础的风险。目前约6%的荷斯坦-弗里生奶牛是一头携带BLAD杂合基因公牛的后代。其后代杂交导致了纯合BLAD犊牛的出生，这些犊牛预期寿命不到1年。BLAD综合征是由编码CD18的基因突变引起的，CD18是白细胞表面β2整合素的一个亚基。通过使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测，目前几个国家正在对大量奶牛进行筛查，以根除突变等位基因。我们描述了一种PCR引物组的优化方法，该方法改进了检测效果。
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