Kostetskiĭ I E, Kirilenko S M, Glazko V I, Sozinov A A
Tsitol Genet. 1996 Mar-Apr;30(2):61-4.
Bovine leukocyte adhesion (BLAD) is a recessive autosomal disease in Holstein-Friesian cattle caused by point mutation in CD18 gene encoding neutrophil-surface glycoprotein. To determine BLAD carriers, the convenient primers were chosen to amplify the mutant region of gene with the following restriction analysis. A screening program for BLAD has been initiated. Among 190 animals from different Ukrainian farms 6 were heterozygous according to the tested trait, i.e., BLAD deficient. No homozygous BLAD carriers were detected.
牛白细胞黏附缺陷症（BLAD）是荷斯坦-弗里生奶牛中的一种常染色体隐性疾病，由编码中性粒细胞表面糖蛋白的CD18基因突变引起。为了确定BLAD携带者，选择了合适的引物扩增该基因的突变区域，并进行以下限制性分析。已启动一项针对BLAD的筛查计划。在来自乌克兰不同农场的190头动物中，有6头根据检测性状为杂合子，即BLAD缺陷型。未检测到纯合BLAD携带者。
