Bogard M, Louvet M, Kamalodine T, Barbanel C
Unité de Biologie Moléculaire, CH de Meaux, France.
Cell Mol Biol (Noisy-le-grand). 1995 Jul;41(5):725-30.
Multicentre quality control studies demonstrated that optimization and standardization of HCV RNA reverse transcription polymerase chain reaction (RT-PCR) amplification techniques were possible: thus, a nested HCV RNA RT-PCR assay was described as a reliable tool for the detection of hepatitis C viral RNA. Besides this, another procedure, the Amplicor HCV RNA qualitative test, a standardized RT-PCR assay, became available. In order to assess the relationship between seropositivity and potential infectivity and to compare both RT-PCR assays, all patients undergoing maintenance hemodialysis were evaluated at hospital de Meaux (Meaux, France) during one year. We conclude that both assays are equally sensitive and that acid guanidinium thiocyanate based methods, which are used to prepare RNA prior to PCR, are more efficient than the usual phenol extraction protocols. Care should be taken with sera from hemodialyzed patients as the presence of inhibitors of the PCR has been demonstrated during the course of HCV RNA testing.
多中心质量控制研究表明，丙型肝炎病毒RNA逆转录聚合酶链反应（RT-PCR）扩增技术的优化和标准化是可行的：因此，巢式丙型肝炎病毒RNA RT-PCR检测方法被描述为检测丙型肝炎病毒RNA的可靠工具。除此之外，另一种方法，即Amplicor HCV RNA定性检测，一种标准化的RT-PCR检测方法，也已问世。为了评估血清阳性与潜在传染性之间的关系，并比较这两种RT-PCR检测方法，法国莫城医院对所有接受维持性血液透析的患者进行了为期一年的评估。我们得出结论，这两种检测方法同样敏感，并且在PCR之前用于制备RNA的基于硫氰酸胍的方法比常用的苯酚提取方法更有效。在检测丙型肝炎病毒RNA的过程中，已证明透析患者的血清中存在PCR抑制剂，因此应对此类血清加以注意。
