Quaresima B, Tiano M T, Porcellini A, D'Agostino P, Faniello M C, Bevilacqua M A, Cimino F, Costanzo F
Dipartimento di Biochimica e Biotecnologie Mediche, Universitá degli Studi di Napoli Federico II, Italia.
PCR Methods Appl. 1994 Oct;4(2):85-8. doi: 10.1101/gr.4.2.85.
The human gene coding for the apoferritin H subunit belongs to a complex multigene family constituted by the expressed gene and by an undefined number of pseudogenes. We have used a strategy based on PCR to amplify specifically the H pseudogenes from a sample of human genomic DNA. With this approach, three new H pseudogenes have been cloned and characterized by DNA sequence analysis. In addition, we have identified a new type of pseudogene, the size of which (700 bp) is caused by multiple detection events in the putative coding region.
