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[Determination of hexokinase isoenzymes in different tissues].

作者信息

Shurda G G, Panin L E

出版信息

Vopr Med Khim. 1978 Mar-Apr;24(2):282-5.

PMID:758095
Abstract

A modified method is described for estimation of hexokinase isoenzymes by polyacrylamide gel electrophoresis. The best conditions for detection of hexokinase activity were developed using immobilization of glucose-6-phosphate dehydrogenas: in gel, utilization of riboflavin as a catalyst of polymerization and stabilization by glucose. The method is simple, highly reproducible, comparatively rapid and economically reasonable.

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