Rutberg M, Billger M, Modig C, Wallin M
Department of Zoophysiology, University of Göteborg, Sweden.
Cell Biol Int. 1995 Sep;19(9):749-58. doi: 10.1006/cbir.1995.1126.
The Atlantic cod (Gadus morhua) is a poikilothermic animal living at temperatures between 2-15 degrees C. Isolated cod brain tubulin is, in contrast to mammalian brain tubulin, posttranslationally modified by acetylation to a high extent. To investigate the role of acetylation in cold adaptation, microtubules were isolated by a taxol-dependent procedure from different organs of the cod, and cells from different tissues were cultured. All cells from skin and brain were able to grow between 4 degrees C and room temperature. Microtubules in the cultured cells were sometimes severed near the periphery of the cells. Microtubules in brain cells were in general more stable to vinblastine and colchicine, when compared to skin cells. Acetylated microtubules were found only in brain cells, in peripheral nerves on scales and in nerves of the intestinal tract and in microtubules isolated from neuronal tissue. Our results show that acetylated microtubules are found both in the central and peripheral nervous system, but that there is no correlation between acetylation and cold-adaptation.
大西洋鳕鱼（Gadus morhua）是一种变温动物，生活在2至15摄氏度的温度环境中。与哺乳动物的脑组织微管蛋白不同，分离出的鳕鱼脑组织微管蛋白在翻译后会大量乙酰化修饰。为了研究乙酰化在冷适应中的作用，通过依赖紫杉醇的方法从鳕鱼的不同器官中分离出微管，并培养来自不同组织的细胞。来自皮肤和大脑的所有细胞都能够在4摄氏度至室温之间生长。培养细胞中的微管有时会在细胞周边附近断裂。与皮肤细胞相比，脑细胞中的微管对长春花碱和秋水仙碱通常更稳定。仅在脑细胞、鳞片上的外周神经、肠道神经以及从神经组织中分离出的微管中发现了乙酰化微管。我们的结果表明，乙酰化微管在中枢和外周神经系统中均有发现，但乙酰化与冷适应之间没有相关性。
