Nichterlein T, Kretschmar M, Mussotter A, Fleischer B, Hof H
Institute of Medical Microbiology and Hygiene, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Mannheim, Germany.
FEMS Immunol Med Microbiol. 1995 Jun;11(3):213-8. doi: 10.1111/j.1574-695X.1995.tb00119.x.
Confrontation of the immune system with bacterial superantigens leads to an initial activation of the immune system followed by a state of profound immunosuppression. To investigate the role of a superantigen in an acute infection with a facultatively intracellular bacterium, we have studied the effect of staphylococcal enterotoxin B on the course of murine listeriosis. Intraperitoneal injection of SEB led to a statistically significant growth restriction of Listeria monocytogenes in the organs of mice infected intravenously or intraperitoneally when treatment with SEB and infection with L. monocytogenes were given simultaneously or when the mice were treated two days before infection. No effect of SEB on murine listeriosis was found when SEB was given more than two days before infection or one or more days after infection. We conclude that initial immunostimulation by SEB which is indicated by a massive liberation of all interleukins measured (IL1 alpha, IL6, TNF alpha, IL2, IFN gamma, IL4) is responsible for the growth restriction of L. monocytogenes in the organs of treated mice. Apoptosis of V beta 8 positive T cells which was accompanied by a 30% reduction of these cells at day 7 after treatment seems to be totally compensated.
免疫系统与细菌超抗原接触会导致免疫系统先被激活，随后进入深度免疫抑制状态。为了研究超抗原在兼性胞内细菌急性感染中的作用，我们研究了葡萄球菌肠毒素B对小鼠李斯特菌病病程的影响。当同时给予SEB处理和感染单核细胞增生李斯特菌，或者在感染前两天对小鼠进行处理时，腹腔注射SEB会导致静脉内或腹腔内感染小鼠器官中单核细胞增生李斯特菌的生长受到统计学上显著的限制。当在感染前超过两天或感染后一天或多天给予SEB时，未发现SEB对小鼠李斯特菌病有影响。我们得出结论，SEB引发的初始免疫刺激（表现为所检测的所有白细胞介素（IL1α、IL6、TNFα、IL2、IFNγ、IL4）大量释放）是导致处理过的小鼠器官中单核细胞增生李斯特菌生长受限的原因。处理后第7天，Vβ8阳性T细胞凋亡，这些细胞数量减少了30%，但似乎完全得到了补偿。
