Institute of Medical Microbiology and Hygiene, Faculty of Clinical Medicine, Mannheim University of Heidelberg, Mannheim.
Inflammopharmacology. 1997;5(4):343-50. doi: 10.1007/s10787-997-0031-8.
Lipoteichoic acids (LTAs) of pathogenic and apathogenic Listeria species and of Staphylococcus aureus were fractionated and tested for their ability to stimulate production of cytokines (IL-1alpha, IL-6, TNF-alpha) in resident peritoneal macrophages (Mvarphi) of endotoxin-resistant C3H/HeJ mice using a serum-free medium. For IL-1alpha and IL-6 there were no detectable differences in the ability of LTA fractions of pathogenic and apathogenic Listeria species and of Staphylococcus aureus. However, LTA-2 fractions of Staphylococcus aureus, which might be less hydrophobic than the LTA-2 fractions of the listeriae-induced lower amounts of TNF-alpha. Furthermore, the more lipophilic LTA-2 fractions of all LTAs employed were more potent inducers of cytokines than the less lipophilic LTA-1 fractions. The biologic effect of LTAs appears, therefore, to depend mainly on their hydrophobicity.
脂磷壁酸（LTAs）的致病性和非致病性李斯特菌和金黄色葡萄球菌进行了分离，并检测其刺激生产细胞因子（IL-1alpha，IL-6，TNF-α）在常驻腹膜巨噬细胞（Mvarphi）的内毒素抵抗 C3H/HeJ 小鼠使用无血清培养基。对于 IL-1alpha 和 IL-6 没有检测到致病性和非致病性李斯特菌和金黄色葡萄球菌的 LTAs 分数的能力有差异。然而，金黄色葡萄球菌的 LTA-2 分数，其可能比李斯特菌诱导的 TNF-α的量低的 LTA-2 分数的疏水性小。此外，所有 LTAs 中疏水性更强的 LTA-2 分数比疏水性更小的 LTA-1 分数更能诱导细胞因子。因此，LTAs 的生物学效应似乎主要取决于其疏水性。
