Ogawa T, Yasuda K, Yamada K, Mori H, Ochiai K, Hasegawa M
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
FEMS Immunol Med Microbiol. 1995 Jun;11(3):247-55. doi: 10.1111/j.1574-695X.1995.tb00122.x.
Mouse monoclonal antibody (mAb) Pgf-II specific for a 72-kDa major cell-surface protein (72K-CSP) derived from Porphyromonas gingivalis OMZ 409 was prepared. Immunoblotting analysis revealed that mAb Pgf-II reacted with 72K-CSP but not with 41-kDa fimbrial subunit protein (41K-fimbrilin) derived from P. gingivalis 381. Electron microscopic observation revealed that P. gingivalis OMZ 409 possessed peritrichous, thin fimbriae on their surface. Immunogold electron microscopy also demonstrated that mAb Pgf-II bound to the 72K-CSP examined with the gold particles arranged along the fibril array originating from the cell surface of the bacteria. These findings suggested that P. gingivalis 72K-CSP was identifiable as another fimbriae (termed Pg-II fimbriae) different from the fimbriae (termed Pg-I fimbriae) composed of a 41K-fimbrilin. Using multipin peptide synthesis technology, 102 sequential overlapping peptides covering the entire 514 amino-acid stretch of Pg-II fimbriae were synthesised. Seven immunodominant regions within Pg-II fimbrial protein molecule, which definitely reacted with the serum of patients with periodontal diseases, were detected.
制备了针对牙龈卟啉单胞菌OMZ 409来源的一种72 kDa主要细胞表面蛋白（72K-CSP）的小鼠单克隆抗体（mAb）Pgf-II。免疫印迹分析显示，mAb Pgf-II与72K-CSP反应，但不与牙龈卟啉单胞菌381来源的41 kDa菌毛亚基蛋白（41K-菌毛蛋白）反应。电子显微镜观察显示，牙龈卟啉单胞菌OMZ 409表面有周毛、细菌毛。免疫金电子显微镜也证明，mAb Pgf-II与用沿源自细菌细胞表面的纤维阵列排列的金颗粒检测的72K-CSP结合。这些发现表明，牙龈卟啉单胞菌72K-CSP可被鉴定为不同于由41K-菌毛蛋白组成的菌毛（称为Pg-I菌毛）的另一种菌毛（称为Pg-II菌毛）。利用多针肽合成技术，合成了覆盖Pg-II菌毛整个514个氨基酸序列的102个连续重叠肽。检测到Pg-II菌毛蛋白分子内7个与牙周病患者血清有明确反应的免疫显性区域。
