利用流式细胞术和数学模型阐明内阿米巴属的DNA合成周期。
Elucidation of the DNA synthetic cycle of Entamoeba spp. using flow cytometry and mathematical modeling.
作者信息
Dvorak J A, Kobayashi S, Alling D W, Hallahan C W
机构信息
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0425, USA.
出版信息
J Eukaryot Microbiol. 1995 Sep-Oct;42(5):610-6. doi: 10.1111/j.1550-7408.1995.tb05915.x.
We developed a method to study the DNA synthetic cycles of Entamoeba histolytica and Entamoeba invadens by flow cytometry (FCM) based on a preparative procedure to reduce both high levels of natural fluorescence and non-specific adsorption of fluorochromes. We modeled G1, S, and G2 phases as a series of overlapping Gaussian curves. Both E. histolytica and E. invadens displayed G1, S, and G2 proportions that are consistent with eukaryotic cell populations in exponential or stationary growth phase. Exponential phase E. histolytica populations contained a hypodiploid subset with a mass of about 20% less than the diploid value which we estimate by FCM to be 24 x 10(-14) g DNA/cell. Exponential phase E. invadens populations contained a hypodiploid subset with a mass of about 6% less than the diploid value which we estimate by FCM to be 30 x 10(-14) g DNA/cell.
我们开发了一种基于流式细胞术（FCM）的方法来研究溶组织内阿米巴和侵袭内阿米巴的DNA合成周期，该方法基于一种制备程序，可减少高水平的自然荧光和荧光染料的非特异性吸附。我们将G1、S和G2期建模为一系列重叠的高斯曲线。溶组织内阿米巴和侵袭内阿米巴的G1、S和G2比例均与处于指数生长期或稳定生长期的真核细胞群体一致。指数生长期的溶组织内阿米巴群体包含一个亚二倍体亚群，其质量比二倍体值低约20%，我们通过FCM估计二倍体值为24×10⁻¹⁴ g DNA/细胞。指数生长期的侵袭内阿米巴群体包含一个亚二倍体亚群，其质量比二倍体值低约6%，我们通过FCM估计二倍体值为30×10⁻¹⁴ g DNA/细胞。
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