Wu J, Pawliszyn J
Department of Chemistry, University of Waterloo, Ont., Canada.
J Chromatogr B Biomed Appl. 1995 Jul 7;669(1):39-43. doi: 10.1016/0378-4347(95)00007-6.
Isoelectric focusing (IEF) was successfully performed in capillary arrays with up to four capillaries. Separated proteins in the capillary array were detected by an UV absorption imaging detector. The whole analysis time for all samples in the capillary array was only 3 min due to the real-time imaging detector. The instrument was applied to analyse several protein samples including different human hemoglobin variants, myoglobin, transferrin, carbonic anhydrase and a monoclonal antibody to fluorescein. Because of good reproducibility of the focused pattern, unknown samples can be run simultaneously with a standard in the multichannel instrument and the components of unknown samples can be identified by comparing their zone positions to those of the standard. Minor components can be determined by the instrument in the presence of major components with 100 times higher concentrations in human hemoglobin samples. This instrument could be a powerful analytical tool for clinical analysis and for quality control in pharmaceutical companies.
等电聚焦（IEF）在多达四根毛细管的毛细管阵列中成功进行。毛细管阵列中分离出的蛋白质通过紫外吸收成像检测器进行检测。由于采用了实时成像检测器，毛细管阵列中所有样品的整个分析时间仅为3分钟。该仪器被应用于分析多种蛋白质样品，包括不同的人类血红蛋白变体、肌红蛋白、转铁蛋白、碳酸酐酶以及一种抗荧光素单克隆抗体。由于聚焦图谱具有良好的重现性，未知样品可在多通道仪器中与标准品同时运行，通过将其区带位置与标准品的区带位置进行比较来鉴定未知样品的成分。在人类血红蛋白样品中，该仪器能够在主要成分浓度比次要成分高100倍的情况下测定次要成分。该仪器可成为临床分析及制药公司质量控制的强大分析工具。
