Wu J, Watson A
Convergent Bioscience Ltd., Toronto, Ont., Canada.
J Chromatogr B Biomed Sci Appl. 1998 Aug 28;714(1):113-8. doi: 10.1016/s0378-4347(98)00036-x.
Sample introduction of an imaged capillary isoelectric focusing (cIEF) instrument is fully automated by using commercially available high-performance liquid chromatography (HPLC) injection valves and autosamplers. Sample carryover can be controlled to under 1% when the valve and separation column are washed for 1 min between sample runs. The standard deviation of peak areas for 20 injections is 3.5%, which includes deviations created by the absorption imaging detector and the isoelectric focusing process inside the 75 microm I.D. column. Sample throughput is up to 10 samples per hour. The instrument has been applied to fast analysis of many proteins including monoclonal antibodies.
成像毛细管等电聚焦（cIEF）仪器的样品引入通过使用市售的高效液相色谱（HPLC）进样阀和自动进样器实现了完全自动化。当在样品运行之间对阀和分离柱进行1分钟的冲洗时，样品残留可控制在1%以下。20次进样的峰面积标准偏差为3.5%，这包括由吸收成像检测器和75微米内径柱内的等电聚焦过程产生的偏差。样品通量高达每小时10个样品。该仪器已应用于包括单克隆抗体在内的多种蛋白质的快速分析。
