Borromeo V, Berrini A, Secchi C, Brambilla G F, Cantafora A
Istituto di Fisiologia Veterinaria e Biochimica, Milan, Italy.
J Chromatogr B Biomed Appl. 1995 Jul 21;669(2):366-71. doi: 10.1016/0378-4347(95)00101-n.
We employed matrix-assisted laser desorption mass spectrometry (LD-MS) to detect recombinant bovine growth hormone (r-bGH) in sustained-release preparations. After preliminary extraction in phosphate buffer, LD-MS provided a precise determination of the molecular mass (M(r)) of the r-bGH contained in 38 sustained-release preparations. The hormone was characterised using enzyme immunoassay, immunoblotting and amino acid sequencing. Rapid detection is essential for analysing large numbers of samples, and for monitoring the use of r-bGH in zootechnical productions and its administration as a "high-tech" drug for therapeutic purposes.
我们采用基质辅助激光解吸质谱法（LD-MS）检测缓释制剂中的重组牛生长激素（r-bGH）。在磷酸盐缓冲液中进行初步提取后，LD-MS可精确测定38种缓释制剂中所含r-bGH的分子量（M(r)）。使用酶免疫测定、免疫印迹和氨基酸测序对该激素进行了表征。对于分析大量样品以及监测r-bGH在畜牧生产中的使用情况及其作为治疗目的的“高科技”药物的给药情况而言，快速检测至关重要。
