Venables W A, Wimpenny J W, Ayres A, Cook S M, Thomas L V
Microbiology Group, School of Pure and Applied Biology, University of Wales Cardiff, UK.
Microbiology (Reading). 1995 Oct;141 ( Pt 10):2713-8. doi: 10.1099/13500872-141-10-2713.
Gel-stabilized two-dimensional gradient plates were used to study the effects of pH, salt concentration and temperature on the conjugal transfer of plasmid RP4 between strains of Escherichia coli and Pseudomonas putida. The combinations of pH and salt concentration that permitted conjugation were mapped as a two-dimensional growth area occupied by transconjugants following conjugation. This conjugation domain was less extensive than the areas that supported growth of the parental strains, and showed evidence for the interactive effects of pH and salt concentration in determination of conditions that permitted conjugation. The size and shape of the conjugation domain was influenced by time, temperature, the identities of the donor and recipient bacteria, and the combination of donor and recipient bacteria.
使用凝胶稳定的二维梯度平板研究pH值、盐浓度和温度对大肠杆菌和恶臭假单胞菌菌株间质粒RP4接合转移的影响。允许接合的pH值和盐浓度组合被绘制为接合后转接合子占据的二维生长区域。该接合域比支持亲本菌株生长的区域范围更小，并显示出pH值和盐浓度在确定允许接合的条件方面存在交互作用的证据。接合域的大小和形状受时间、温度、供体菌和受体菌的种类以及供体菌与受体菌组合的影响。
