Holben W E, Harris D
Centre for Microbial Ecology, Michigan State University, East Lansing 48824, USA.
Mol Ecol. 1995 Oct;4(5):627-31. doi: 10.1111/j.1365-294x.1995.tb00263.x.
Determining the structure of bacterial communities and their response to stimuli is key to understanding community function and the interactions that occur between micro-organisms and the environment. However, bacterial communities often comprise complex assemblages of large numbers of different bacterial populations. An approach is presented which allows bacterial community structure to be determined by fractionation of the complex mixture of total bacterial community DNA using the DNA-binding dye bisbenzimidazole which imposes G+C-dependent changes in the buoyant density of DNA. Bacterial community structure presented as percentage of total DNA vs. percentage G+C content of DNA is an indication of the relative abundance of phylogenetic groups of bacteria. Changes in the composition of a soil bacterial community in response to perturbations in the form of carbon amendment and altered water status were monitored.
确定细菌群落的结构及其对刺激的反应是理解群落功能以及微生物与环境之间相互作用的关键。然而，细菌群落通常由大量不同细菌种群的复杂组合构成。本文提出了一种方法，该方法通过使用DNA结合染料双苯并咪唑对总细菌群落DNA的复杂混合物进行分级分离来确定细菌群落结构，双苯并咪唑会使DNA的浮力密度发生G+C依赖性变化。以总DNA百分比与DNA的G+C含量百分比表示的细菌群落结构是细菌系统发育群体相对丰度的一个指标。监测了土壤细菌群落组成因碳添加和水分状况改变等扰动形式而发生的变化。
