Woolley M J, Lane C G, Ellis R, Stevens W H, Woolley K L, O'Byrne P M
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Am J Respir Crit Care Med. 1995 Nov;152(5 Pt 1):1508-12. doi: 10.1164/ajrccm.152.5.7582285.
The role of the eosinophil in the development of allergen-induced airway hyperresponsiveness is uncertain. We examined whether the development of airway hyperresponsiveness in 17 dogs after inhalation of Ascaris suum allergen (10(-6) to 10(-2) weight/volume [w/v]) was associated with increases in the number and level of activation of eosinophils before and after allergen inhalation. Airway responsiveness to inhaled acetylcholine was measured before and 24 h after Ascaris inhalation. Eosinophil number was assessed by bronchoalveolar lavage performed 1 wk before allergen inhalation and 15 min after the 24 h acetylcholine challenge. Dogs that developed Ascaris-induced airway hyperresponsiveness (n = 8) had a significantly greater number of bronchoalveolar lavage eosinophils before allergen inhalation (mean +/- SEM: 4.6 +/- 1.94 x 10(4) cells/ml) than dogs that did not become hyperresponsive (n = 9) (1.2 +/- 0.81 x 10(4) cells/ml) (p = 0.03). Ascaris-induced airway hyperresponsiveness, measured 24 h after allergen inhalation, was not associated with increases in eosinophil number after allergen challenge. These results suggest that the presence of airway eosinophils before allergen inhalation is necessary for the development of allergen-induced airway hyperresponsiveness.
嗜酸性粒细胞在变应原诱导的气道高反应性发展中的作用尚不确定。我们研究了17只犬吸入猪蛔虫变应原（10^(-6)至10^(-2)重量/体积[w/v]）后气道高反应性的发展是否与变应原吸入前后嗜酸性粒细胞数量及活化水平的增加相关。在吸入猪蛔虫变应原之前和之后24小时测量气道对吸入乙酰胆碱的反应性。通过在变应原吸入前1周以及24小时乙酰胆碱激发后15分钟进行支气管肺泡灌洗来评估嗜酸性粒细胞数量。出现猪蛔虫诱导的气道高反应性的犬（n = 8）在变应原吸入前支气管肺泡灌洗嗜酸性粒细胞数量（平均值±标准误：4.6±1.94×10^4个细胞/ml）显著高于未出现高反应性的犬（n = 9）（1.2±0.81×10^4个细胞/ml）（p = 0.03）。变应原吸入后24小时测量的猪蛔虫诱导的气道高反应性与变应原激发后嗜酸性粒细胞数量的增加无关。这些结果表明，变应原吸入前气道嗜酸性粒细胞的存在是变应原诱导的气道高反应性发展所必需的。
