Ayadi A, Dutoit E, Sendid B, Camus D
Laboratorie de Parasitologie-Mycologie, Faculté de Médecine, Sfax, Tunisie.
Parasite. 1995 Jun;2(2):119-23. doi: 10.1051/parasite/1995022119.
A western blot assay was performed for the detection of Echinococcus granulosus specific antigens useful for the diagnostic of hydatic disease. 191 sera were tested, 105 coming from patients with different localizations of hydatic cysts and 86 from persons either healthy or presenting other diseases. 48 different antigenic bands were detected using sera from patients with hydatidosis. A 35 kDa antigen co-migrating with a band labeled by a McAb specific of antigen 5 was recognized in western blot by only 68% of the sera able to precipitate antigen 5 in immunoelectrophoresis. A 8 kDa antigen corresponding to the specific E. granulosus antigen already described has been recognized by 80% of the sera coming from patients with hydatidosis and not by the 86 control sera. Bands of 21, 30, and 92 kDa appeared also specific and were recognized by at least 50% of tested sera. These antigens appeared unrelated one to each other. 103 out of the 105 sera from patients with hydatidosis were able to recognize at least one of the 8, 21, 30, 35 or 92 kDa specific antigens. The present results suggest that western blot could be useful for the diagnosis of hydatidosis as far as the criteria of positivity is based on the recognition of at least one of the major specific antigens.
进行了蛋白质印迹分析，以检测对包虫病诊断有用的细粒棘球绦虫特异性抗原。检测了191份血清，其中105份来自患有不同部位包虫囊肿的患者，86份来自健康人或患有其他疾病的人。使用来自包虫病患者的血清检测到48条不同的抗原带。在蛋白质印迹中，仅68%能够在免疫电泳中沉淀抗原5的血清识别出一条与抗原5特异性单克隆抗体标记带共迁移的35 kDa抗原。一种对应于已描述的细粒棘球绦虫特异性抗原的8 kDa抗原已被80%来自包虫病患者的血清识别，而86份对照血清未识别。21、30和92 kDa的条带也显示出特异性，并且至少50%的检测血清识别出这些条带。这些抗原彼此之间似乎没有关联。105份来自包虫病患者的血清中有103份能够识别8、21、30、35或92 kDa特异性抗原中的至少一种。目前的结果表明，只要阳性标准基于识别至少一种主要特异性抗原，蛋白质印迹法可用于包虫病的诊断。
