血小板衍生生长因子对兔离体耳动脉细胞电压门控钙通道的影响。

Effect of platelet-derived growth factor on voltage-operated calcium channels in rabbit isolated ear artery cells.

Wijetunge S, Hughes A D

Department of Clinical Pharmacology, St. Mary's Hospital Medical School, Imperial College of Science Technology & Medicine, London.

Br J Pharmacol. 1995 Jun;115(3):534-8. doi: 10.1111/j.1476-5381.1995.tb16367.x.

Abstract

Platelet derived growth factor (PDGF), AB and BB isoforms (100 pM) increased calcium channel currents measured by whole cell voltage clamp technique in single vascular smooth muscle cells isolated from rabbit ear arteries. 2. Tyrphostin-23 (100 microM) a selective inhibitor of protein tyrosine kinases, reduced calcium channel currents. Pre-incubation with tyrphostin-23 prevented PDGF-AB induced increase in calcium channel currents. However, in these same cells 10 nM (+)-202791, a dihydropyridine calcium channel agonist, did increase calcium channel currents. 3. Bistyrphostin (10 microM), a selective inhibitor of epidermal growth factor (EGF)-kinase also reduced calcium channel currents and inhibited PDGF-AB-induced increases in calcium channel currents. 4. Genistein (100 microM) a selective inhibitor of tyrosine kinases, structurally unrelated to the tryphostins, also inhibited calcium channel currents and pre-incubation with genistein prevented the PDGF-AB-induced rise in calcium channel currents. 5. These results indicate that PDGF increases calcium channel currents in vascular smooth muscle. This action of PDGF probably involves a tyrosine kinase.

摘要

血小板衍生生长因子（PDGF）的AB和BB亚型（100皮摩尔），通过全细胞电压钳技术测定，可增加从兔耳动脉分离出的单个血管平滑肌细胞中的钙通道电流。2. 酪氨酸磷酸化抑制剂-23（100微摩尔），一种蛋白酪氨酸激酶的选择性抑制剂，可降低钙通道电流。预先用酪氨酸磷酸化抑制剂-23孵育可阻止PDGF-AB诱导的钙通道电流增加。然而，在这些相同的细胞中，10纳摩尔（+）-202791，一种二氢吡啶钙通道激动剂，确实增加了钙通道电流。3. 双酪氨酸磷酸化抑制剂（10微摩尔），一种表皮生长因子（EGF）激酶的选择性抑制剂，也降低了钙通道电流，并抑制了PDGF-AB诱导的钙通道电流增加。4. 染料木黄酮（100微摩尔），一种与酪氨酸磷酸化抑制剂结构无关的酪氨酸激酶选择性抑制剂，也抑制了钙通道电流，预先用染料木黄酮孵育可阻止PDGF-AB诱导的钙通道电流升高。5. 这些结果表明，PDGF增加血管平滑肌中的钙通道电流。PDGF的这种作用可能涉及一种酪氨酸激酶。