血小板衍生生长因子对兔离体耳动脉细胞电压门控钙通道的影响。
Effect of platelet-derived growth factor on voltage-operated calcium channels in rabbit isolated ear artery cells.
作者信息
Wijetunge S, Hughes A D
机构信息
Department of Clinical Pharmacology, St. Mary's Hospital Medical School, Imperial College of Science Technology & Medicine, London.
出版信息
Br J Pharmacol. 1995 Jun;115(3):534-8. doi: 10.1111/j.1476-5381.1995.tb16367.x.
Abstract
- Platelet derived growth factor (PDGF), AB and BB isoforms (100 pM) increased calcium channel currents measured by whole cell voltage clamp technique in single vascular smooth muscle cells isolated from rabbit ear arteries. 2. Tyrphostin-23 (100 microM) a selective inhibitor of protein tyrosine kinases, reduced calcium channel currents. Pre-incubation with tyrphostin-23 prevented PDGF-AB induced increase in calcium channel currents. However, in these same cells 10 nM (+)-202791, a dihydropyridine calcium channel agonist, did increase calcium channel currents. 3. Bistyrphostin (10 microM), a selective inhibitor of epidermal growth factor (EGF)-kinase also reduced calcium channel currents and inhibited PDGF-AB-induced increases in calcium channel currents. 4. Genistein (100 microM) a selective inhibitor of tyrosine kinases, structurally unrelated to the tryphostins, also inhibited calcium channel currents and pre-incubation with genistein prevented the PDGF-AB-induced rise in calcium channel currents. 5. These results indicate that PDGF increases calcium channel currents in vascular smooth muscle. This action of PDGF probably involves a tyrosine kinase.
摘要
- 血小板衍生生长因子（PDGF）的AB和BB亚型（100皮摩尔），通过全细胞电压钳技术测定，可增加从兔耳动脉分离出的单个血管平滑肌细胞中的钙通道电流。2. 酪氨酸磷酸化抑制剂-23（100微摩尔），一种蛋白酪氨酸激酶的选择性抑制剂，可降低钙通道电流。预先用酪氨酸磷酸化抑制剂-23孵育可阻止PDGF-AB诱导的钙通道电流增加。然而，在这些相同的细胞中，10纳摩尔（+）-202791，一种二氢吡啶钙通道激动剂，确实增加了钙通道电流。3. 双酪氨酸磷酸化抑制剂（10微摩尔），一种表皮生长因子（EGF）激酶的选择性抑制剂，也降低了钙通道电流，并抑制了PDGF-AB诱导的钙通道电流增加。4. 染料木黄酮（100微摩尔），一种与酪氨酸磷酸化抑制剂结构无关的酪氨酸激酶选择性抑制剂，也抑制了钙通道电流，预先用染料木黄酮孵育可阻止PDGF-AB诱导的钙通道电流升高。5. 这些结果表明，PDGF增加血管平滑肌中的钙通道电流。PDGF的这种作用可能涉及一种酪氨酸激酶。
相似文献
1
Effect of platelet-derived growth factor on voltage-operated calcium channels in rabbit isolated ear artery cells.血小板衍生生长因子对兔离体耳动脉细胞电压门控钙通道的影响。
Br J Pharmacol. 1995 Jun;115(3):534-8. doi: 10.1111/j.1476-5381.1995.tb16367.x.
2
Tyrosine kinase inhibitors block calcium channel currents in vascular smooth muscle cells.酪氨酸激酶抑制剂可阻断血管平滑肌细胞中的钙通道电流。
Biochem Biophys Res Commun. 1992 Dec 30;189(3):1620-3. doi: 10.1016/0006-291x(92)90262-j.
3
Effects of protein tyrosine kinase inhibitors on voltage-operated calcium channel currents in vascular smooth muscle cells and pp60(c-src) kinase activity.蛋白酪氨酸激酶抑制剂对血管平滑肌细胞电压门控钙通道电流及pp60(c-src)激酶活性的影响。
Br J Pharmacol. 2000 Apr;129(7):1347-54. doi: 10.1038/sj.bjp.0703186.
4
Effect of inhibition of tyrosine phosphatases on voltage-operated calcium channel currents in rabbit isolated ear artery cells.酪氨酸磷酸酶抑制对兔离体耳动脉细胞电压门控钙通道电流的影响。
Br J Pharmacol. 1998 May;124(2):307-16. doi: 10.1038/sj.bjp.0701840.
5
Increase in tone and intracellular Ca2+ in rabbit isolated ear artery by platelet-derived growth factor.血小板衍生生长因子使兔离体耳动脉张力和细胞内钙离子增加。
Br J Pharmacol. 1995 Jan;114(1):138-42. doi: 10.1111/j.1476-5381.1995.tb14917.x.
6
7
Modulation of Ca(2+)-activated K+ channel activity by tyrosine kinase inhibitors in vascular smooth muscle cell.酪氨酸激酶抑制剂对血管平滑肌细胞中钙激活钾通道活性的调节作用
Eur J Pharmacol. 1995 Jul 18;290(2):117-23. doi: 10.1016/0922-4106(95)90023-3.
8
Inhibitory effects of genistein on ATP-sensitive K+ channels in rabbit portal vein smooth muscle.染料木黄酮对兔门静脉平滑肌中ATP敏感性钾通道的抑制作用。
Br J Pharmacol. 1997 Dec;122(7):1395-404. doi: 10.1038/sj.bjp.0701532.
9
Tyrphostins inhibit PDGF-induced DNA synthesis and associated early events in smooth muscle cells.酪氨酸磷酸化抑制剂可抑制血小板衍生生长因子（PDGF）诱导的平滑肌细胞DNA合成及相关早期事件。
Am J Physiol. 1991 Apr;260(4 Pt 1):C721-30. doi: 10.1152/ajpcell.1991.260.4.C721.
10
Specific inhibition of stretch-induced increase in L-type calcium channel currents by herbimycin A in canine basilar arterial myocytes.赫伯霉素A对犬基底动脉肌细胞中牵张诱导的L型钙通道电流增加的特异性抑制作用。
Br J Pharmacol. 2000 Jun;130(4):923-31. doi: 10.1038/sj.bjp.0703360.
引用本文的文献
1
Imatinib relaxes the pulmonary venous bed of guinea pigs.伊马替尼可舒张豚鼠的肺静脉床。
Respir Res. 2017 Feb 8;18(1):32. doi: 10.1186/s12931-017-0514-0.
2
Insensitivity of cardiac delayed-rectifier I(Kr) to tyrosine phosphorylation inhibitors and stimulators.心脏延迟整流钾电流（I(Kr)）对酪氨酸磷酸化抑制剂和刺激剂不敏感。
Br J Pharmacol. 2006 Jul;148(5):724-31. doi: 10.1038/sj.bjp.0706776. Epub 2006 May 22.
3
Lysophosphatidylcholine potentiates vascular contractile responses in rat aorta via activation of tyrosine kinase.溶血磷脂酰胆碱通过激活酪氨酸激酶增强大鼠主动脉的血管收缩反应。
Br J Pharmacol. 2002 Feb;135(3):789-99. doi: 10.1038/sj.bjp.0704525.
4
Tyrosine kinase involvement in renal arteriolar constrictor responses to angiotensin II.酪氨酸激酶参与肾小动脉对血管紧张素II的收缩反应。
Hypertension. 2001 Feb;37(2 Pt 2):569-73. doi: 10.1161/01.hyp.37.2.569.
5
Smooth muscle contractility and protein tyrosine phosphorylation.平滑肌收缩性与蛋白质酪氨酸磷酸化
Mol Cell Biochem. 1997 Nov;176(1-2):47-51.
本文引用的文献
1
Vascular derived growth factors: cell biology, pathophysiology, and pharmacology.血管衍生生长因子：细胞生物学、病理生理学及药理学
Pharmacol Rev. 1993 Mar;45(1):1-42.
2
Effects of tyrosine kinase inhibitors on protein kinase-independent systems.酪氨酸激酶抑制剂对非蛋白激酶系统的作用。
FEBS Lett. 1993 Feb 1;316(3):278-82. doi: 10.1016/0014-5793(93)81308-m.
3
The acute actions of growth factors in smooth muscle systems.生长因子在平滑肌系统中的急性作用。
Life Sci. 1994;54(4):223-35. doi: 10.1016/0024-3205(94)00811-6.
4
5
How receptor tyrosine kinases activate Ras.受体酪氨酸激酶如何激活Ras。
Trends Biochem Sci. 1993 Aug;18(8):273-5. doi: 10.1016/0968-0004(93)90031-h.
6
7
Tyrosine kinase pathways and the regulation of smooth muscle contractility.酪氨酸激酶途径与平滑肌收缩性的调节
Trends Pharmacol Sci. 1994 Apr;15(4):108-14. doi: 10.1016/0165-6147(94)90046-9.
8
Increase in tone and intracellular Ca2+ in rabbit isolated ear artery by platelet-derived growth factor.血小板衍生生长因子使兔离体耳动脉张力和细胞内钙离子增加。
Br J Pharmacol. 1995 Jan;114(1):138-42. doi: 10.1111/j.1476-5381.1995.tb14917.x.
9
Channel regulation. Ion channel control by tyrosine phosphorylation.通道调节。酪氨酸磷酸化对离子通道的调控。
Curr Biol. 1994 Mar 1;4(3):242-5. doi: 10.1016/s0960-9822(00)00054-3.
Zotero 插件