Roivainen A, Isomäki P, Nikkari S, Saario R, Vuori K, Toivanen P
Department of Medical Microbiology, Turku University, Finland.
Br J Rheumatol. 1995 Sep;34(9):805-8. doi: 10.1093/rheumatology/34.9.805.
Since it has been implied that cellular oncogenes might have a role in the pathogenesis of rheumatoid arthritis (RA), we have examined the expression of c-myc, c-myb, c-fos, c-jun and c-Ha-ras oncogenes in the cells from synovial fluid (SF) and peripheral blood (PB) of patients with reactive arthritis (ReA) and early RA. Oncogene expression was studied using RNA hybridizations with 32P-labelled probes. From the SF, mononuclear and granulocyte cell fractions were used separately. Significant differences between ReA and RA were observed only for c-myb in PB mononuclear cells and c-jun in SF granulocytes. Regarding the expression of c-myc, c-fos and c-Ha-ras oncogenes, no difference between ReA and RA was observed. Comparison to normal controls was made using PB mononuclear cells; only the expression of c-fos tended to be slightly increased in RA, without statistical significance, however. We conclude that oncogene activation in the synovial inflammation is not a phenomenon specific for RA.
由于已经暗示细胞癌基因可能在类风湿性关节炎（RA）的发病机制中起作用，我们研究了反应性关节炎（ReA）和早期RA患者滑液（SF）和外周血（PB）细胞中c-myc、c-myb、c-fos、c-jun和c-Ha-ras癌基因的表达。使用32P标记的探针通过RNA杂交研究癌基因表达。从SF中分别使用单核细胞和粒细胞组分。仅在PB单核细胞中的c-myb和SF粒细胞中的c-jun观察到ReA和RA之间的显著差异。关于c-myc、c-fos和c-Ha-ras癌基因的表达，未观察到ReA和RA之间的差异。使用PB单核细胞与正常对照进行比较；然而，仅RA中c-fos的表达有轻微增加的趋势，但无统计学意义。我们得出结论，滑膜炎症中的癌基因激活不是RA特有的现象。
