Abogadie F C, Bruch R C, Farbman A I
Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208, USA.
Chem Senses. 1995 Apr;20(2):199-206. doi: 10.1093/chemse/20.2.199.
Olfactory signal transduction in a number of species has been shown to be mediated by heterotrimeric GTP-binding proteins (G-proteins). The expression of different G-proteins in channel catfish (Ictalurus punctatus) olfactory epithelium was investigated using antibodies to both the alpha and beta subunits of G-proteins. Based on Western blotting and immunohistochemical data, the following G-protein subunits were identified in the olfactory epithelium: Gs/G(olf), Gi1, Gi2, Gq and G beta. Immunohistochemical results indicated that all of these G-proteins, encompassing three G-protein subfamilies, were expressed in the dendrites and cilia of olfactory receptor neurons. These findings suggest that different G-protein subunits may mediate multiple signal transduction pathways in the catfish olfactory system, i.e. G(olf)/Gs, may mediate odorant activation of adenylyl cyclase while Gi and G beta may mediate odorant activation of phospholipase C.
在许多物种中，嗅觉信号转导已被证明是由异源三聚体GTP结合蛋白（G蛋白）介导的。使用针对G蛋白α和β亚基的抗体，研究了斑点叉尾鮰嗅觉上皮中不同G蛋白的表达。基于蛋白质免疫印迹和免疫组织化学数据，在嗅觉上皮中鉴定出以下G蛋白亚基：Gs/G(olf)、Gi1、Gi2、Gq和Gβ。免疫组织化学结果表明，所有这些G蛋白，包括三个G蛋白亚家族，均在嗅觉受体神经元的树突和纤毛中表达。这些发现表明，不同的G蛋白亚基可能介导鲶鱼嗅觉系统中的多种信号转导途径，即G(olf)/Gs可能介导腺苷酸环化酶的气味激活，而Gi和Gβ可能介导磷脂酶C的气味激活。
