Dellacorte C, Restrepo D, Menco B P, Andreini I, Kalinoski D L
Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
Neuroscience. 1996 Sep;74(1):261-73. doi: 10.1016/0306-4522(96)00115-7.
The immunohistochemical localization of G alpha 9/G alpha 11 was studied in the olfactory and respiratory epithelium of two representative vertebrates, the rat and the channel catfish. Localization in the rat was found at the apical surface of cells in the epithelium and within nerve tracts in the lamina propria. Immunostaining of neuronal cilia and supporting cell microvilli was confirmed by electron microscopy. Immunoreactivity on the ipsilateral neuroepithelium was abolished five weeks following unilateral bulbectomy. An emergence of patchy immunoreactivity was found, however, after fifteen weeks. In catfish, G alpha 9/G alpha 11 antigenicity was found at the apical surface of cells within the olfactory epithelium, at supranuclear regions within some cell bodies and in basal nerve tracts of the olfactory rosette. Immunoreactivity was removed with unilateral bulbectomy. Specific labelling in both rat and catfish was eliminated by preincubation of the G alpha 9/G alpha 11 antibodies with the cognate peptide. Proteins were extracted from olfactory tissues of both species and solubilized. Using western blotting, bands corresponding in apparent molecular weight to a 38,000 mol. wt protein were found. These data demonstrate the presence of G alpha 9/G alpha 11 in the olfactory tissues of these vertebrates and suggest a role in olfaction for this class of G-protein.
在两种具有代表性的脊椎动物——大鼠和斑点叉尾鮰的嗅觉和呼吸上皮中，研究了Gα9 / Gα11的免疫组织化学定位。在大鼠中，其定位见于上皮细胞的顶端表面以及固有层的神经束内。通过电子显微镜证实了神经元纤毛和支持细胞微绒毛上的免疫染色。单侧嗅球切除术后五周，同侧神经上皮的免疫反应性消失。然而，十五周后发现出现了斑片状免疫反应性。在斑点叉尾鮰中，Gα9 / Gα11抗原性见于嗅觉上皮细胞的顶端表面、一些细胞体的核上区域以及嗅叶的基底神经束。单侧嗅球切除可消除免疫反应性。用Gα9 / Gα11抗体与同源肽预孵育可消除大鼠和斑点叉尾鮰中的特异性标记。从这两个物种的嗅觉组织中提取并溶解蛋白质。使用蛋白质印迹法，发现了表观分子量与38,000摩尔质量蛋白质相对应的条带。这些数据证明了这些脊椎动物的嗅觉组织中存在Gα9 / Gα11，并表明这类G蛋白在嗅觉中起作用。
