Grkovic I, Anderson C R
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria, Australia.
Brain Res. 1995 Jul 3;684(2):127-35. doi: 10.1016/0006-8993(95)00392-4.
The distribution and targets of calretinin-immunoreactive preganglionic nerve terminals in the superior cervical ganglion of the rat were examined using immunohistochemistry and retrograde neuronal tracing. Calretinin-immunoreactive nerve terminals were found throughout the ganglion, forming distinct pericellular baskets around a sub-population of postganglionic neurons. The targets of postganglionic neurons surrounded by calretinin-immunoreactive nerve terminals were determined after injection of tracer into the submandibular salivary gland, the extra-orbital lacrimal gland, the thyroid gland, the anterior chamber of the eye or the skin of the forehead. Only when tracer was injected into the submandibular gland were neurons labelled that were surrounded by calretinin-immunoreactive nerve terminals. When immunohistochemistry using antisera to neuropeptide Y was combined with retrograde tracing, only submandibular gland projecting neurons lacking neuropeptide Y were surrounded by calretinin-immunoreactive terminals. When retrograde neuronal tracer was injected into the superior cervical ganglion, a proportion of retrogradely-labelled neurons in the upper thoracic spinal cord showed relatively weak calretinin-immunoreactivity. All calretinin-immunoreactive terminals in the superior cervical ganglion disappeared following section of the sympathetic chain distal to the superior cervical ganglion. Thus, calretinin is present in a population of preganglionic neurons projecting exclusively to neuropeptide Y non-immunoreactive (presumably secretomotor) neurons innervating the submandibular salivary gland of the rat.
采用免疫组织化学和逆行神经元示踪技术，研究了大鼠颈上神经节中钙视网膜蛋白免疫反应性节前神经终末的分布和靶标。在整个神经节中均发现了钙视网膜蛋白免疫反应性神经终末，它们在节后神经元的一个亚群周围形成明显的细胞周围篮状结构。在将示踪剂注入下颌下唾液腺、眶外泪腺、甲状腺、眼前房或前额皮肤后，确定了被钙视网膜蛋白免疫反应性神经终末包围的节后神经元的靶标。仅当示踪剂注入下颌下腺时，被钙视网膜蛋白免疫反应性神经终末包围的神经元才被标记。当使用抗神经肽Y抗血清的免疫组织化学与逆行示踪相结合时，只有缺乏神经肽Y的下颌下腺投射神经元被钙视网膜蛋白免疫反应性终末包围。当将逆行神经元示踪剂注入颈上神经节时，上胸段脊髓中一部分逆行标记的神经元显示出相对较弱的钙视网膜蛋白免疫反应性。在颈上神经节远端的交感神经链切断后，颈上神经节中所有的钙视网膜蛋白免疫反应性终末均消失。因此，钙视网膜蛋白存在于一群节前神经元中，这些节前神经元仅投射到支配大鼠下颌下唾液腺的神经肽Y非免疫反应性（可能是分泌运动性）神经元。
