Blomgren K, Kawashima S, Saido T C, Karlsson J O, Elmered A, Hagberg H
Institute of Anatomy and Cell Biology, Göteborg University, Sweden.
Brain Res. 1995 Jul 3;684(2):143-9. doi: 10.1016/0006-8993(95)00399-b.
Neonatal rats were subjected to transient cerebral hypoxic-ischemia (unilateral occlusion of the common carotid artery + 7.70% O2 for 100 min). Ipsi-and contralateral parietal cerebral cortex was assayed with Western blotting for fodrin breakdown product (FBDP). Calpain immunoreactivity was assayed in the cytosolic fraction (CF) and the membrane and microsomal fraction (MMF). Calpain immunoreactivity decreased bilaterally in the CF during the insult (62-68% of controls) and remained significantly lower during early recovery, whereas the MMF showed no significant changes. This relative redistribution of calpains coincided with the appearance of FBDP in the left, ipsilateral hemisphere, displaying a significantly higher level of FBDP from immediately after the insult until at least 1 day of recovery (204-292% of controls). No significant changes in FBDP could be detected in the right, contralateral hemisphere, indicating that although redistribution of calpains occurred, hypoxia per se did not suffice to initiate fodrin degradation in this model of neonatal hypoxic-ischemia.
将新生大鼠置于短暂性脑缺氧缺血环境（单侧颈总动脉闭塞 + 7.70%氧气，持续100分钟）。通过蛋白质免疫印迹法检测同侧和对侧顶叶大脑皮质的血影蛋白降解产物（FBDP）。在胞质部分（CF）以及膜和微粒体部分（MMF）检测钙蛋白酶免疫反应性。在损伤期间，CF中的钙蛋白酶免疫反应性双侧降低（为对照组的62 - 68%），并且在早期恢复期间仍显著较低，而MMF未显示出显著变化。钙蛋白酶的这种相对重新分布与左侧同侧半球中FBDP的出现同时发生，从损伤后即刻直至至少恢复1天，FBDP水平显著更高（为对照组的204 - 292%）。在右侧对侧半球未检测到FBDP有显著变化，这表明尽管发生了钙蛋白酶的重新分布，但在该新生缺氧缺血模型中，缺氧本身不足以启动血影蛋白降解。
