Luo Y, Peng N, Yang W, Zhang W
Department of Anatomy, Guangxi Medical University, Nanning, People's Republic of China.
Brain Res. 1995 Jul 31;687(1-2):191-3. doi: 10.1016/0006-8993(95)00381-y.
The distribution of vasopressin (VP)-immunoreactive neuronal perikarya and its fibers had been studied in the hypothalamus of Tupaia belangeri using the avidin-biotin complex (ABC) immunocytochemical technique. VP-immunoreactive neurons were found in the hypothalamic paraventricular nucleus (PVN), supraoptic nucleus (SON), accessory supraoptic nucleus (ASN), hypothalamic lateral nucleus (HLN), perifornical nucleus (PFN) and ansa peduncularis (AP) but not in the suprachiasmatic nucleus (SCN). VP neurons of the rostral PVN could be divided into three subnuclei and the caudal PVN could also be divided although not so distinctly into four subnuclei. The VP-immunoreactive neuronal perikarya of SON were divided into three parts, i.e., medioventral, mediodorsal and laterodorsal. Three types of VP neuronal perikarya, i.e., large, medium and small cells, existed in PVN and SON. Between PVN and SON, there were a large number of VP immunopositive nerve fibers. In addition, there were numerous immunopositive fibers projecting into the infundibulum and the neurohypophysis. VP-immunoreactive-positive products localized in the large granular vesicles and on the rough-surfaced endoplasmic reticulum could be seen under electron microscope.
采用抗生物素蛋白-生物素复合物（ABC）免疫细胞化学技术，研究了树鼩下丘脑内加压素（VP）免疫反应性神经元胞体及其纤维的分布。在下丘脑室旁核（PVN）、视上核（SON）、视上旁核（ASN）、下丘脑外侧核（HLN）、穹窿周核（PFN）和脚间袢（AP）中发现了VP免疫反应性神经元，而在视交叉上核（SCN）中未发现。PVN前部的VP神经元可分为三个亚核，PVN后部也可分为四个亚核，但不如前部明显。SON的VP免疫反应性神经元胞体分为三部分，即内侧腹侧、内侧背侧和外侧背侧。PVN和SON中存在三种类型的VP神经元胞体，即大、中、小细胞。在PVN和SON之间，有大量的VP免疫阳性神经纤维。此外，有许多免疫阳性纤维投射到漏斗和神经垂体。在电子显微镜下可见，VP免疫反应阳性产物定位于大颗粒囊泡和粗面内质网上。
