Sasaki K, Oomura Y, Figurov A, Morita N, Yanaihara N
Division of Bio-Information Engineering, Faculty of Engineering, Toyama University, Japan.
Brain Res Bull. 1995;38(2):185-91. doi: 10.1016/0361-9230(95)00092-s.
Effects of acidic fibroblast growth factor (aFGF) fragments such as aminoterminal aFGF (1-15) and carboxyl-terminal aFGF (114-140) on synaptic transmission were investigated in rat hippocampal slices. Stimulation was applied to Schaffer collateral/commissural afferents, and evoked population spikes were recorded in the CA1 pyramidal cell layer. Continuous perfusion of slices with aFGF (1-15) slightly decreased the basal amplitude of population spikes and significantly increased the paired-pulse facilitation. When brief tetanic stimulation (7 pulses at 100 Hz) was applied 30 min after the perfusion of aFGF (1-15), aFGF (1-15)-treated slices enhanced the magnitude of short-term potentiation after the tetanus and facilitated a generation of long-term potentiation. These effects of aFGF (1-15) were dose-dependent. Perfusion of slices with aFGF (114-140) had no effect on the basal spike amplitude, paired-pulse facilitation, and short-term potentiation. Both aFGF (1-15) and aFGF (114-140) had no effect on the DNA synthesis-stimulating activity in BALB/c 3T3-L1 cells. The results suggest that aFGF (1-15), which is not involved in mitogenic activity, is implicated in a modulatory mechanism of synaptic plasticity.
在大鼠海马脑片中研究了酸性成纤维细胞生长因子（aFGF）片段如氨基末端aFGF（1 - 15）和羧基末端aFGF（114 - 140）对突触传递的影响。刺激施加于Schaffer侧支/连合传入纤维，并在CA1锥体细胞层记录诱发的群体峰电位。用aFGF（1 - 15）持续灌流脑片会轻微降低群体峰电位的基础幅度，并显著增加双脉冲易化。在灌流aFGF（1 - 15）30分钟后施加短暂强直刺激（100 Hz，7个脉冲）时，经aFGF（1 - 15）处理的脑片在强直刺激后增强了短期增强的幅度，并促进了长期增强的产生。aFGF（1 - 15）的这些作用呈剂量依赖性。用aFGF（114 - 140）灌流脑片对基础峰电位幅度、双脉冲易化和短期增强没有影响。aFGF（1 - 15）和aFGF（114 - 140）对BALB/c 3T3 - L1细胞中的DNA合成刺激活性均无影响。结果表明，不参与有丝分裂活性的aFGF（1 - 15）与突触可塑性的调节机制有关。
