Sasaki K, Oomura Y, Figurov A, Yagi H
Department of Electronics and Computer Science, Faculty of Engineering, Toyama University, Japan.
Brain Res Bull. 1994;33(5):505-11. doi: 10.1016/0361-9230(94)90075-2.
In the present study, effects of acidic fibroblast growth factor (aFGF, 0.5-2.5 ng/ml) on synaptic transmission were investigated in rat hippocampal slices. Stimulation was applied to Schaffer collateral/commissural afferents and evoked spikes were recorded in CA1 pyramidal cell layer. Continuous perfusion of slices with aFGF slightly decreased the basal amplitude of the spikes and significantly increased the paired-pulse facilitation. When brief tetanic stimulation (7 impulses at 100 Hz) was applied 30 min after the perfusion of aFGF, aFGF-treated slices enhanced the magnitude of short-term potentiation after the tetanus and facilitated the generation of long-term potentiation. aFGF also enhanced post-tetanic potentiation directly after the tetanus. These effects of aFGF were dose-dependent. The enhancement of short-term potentiation and facilitation of the generation of long-term potentiation were not evident when aFGF was applied with or 10 min after the tetanus. The results suggest that aFGF is implicated in modulation of synaptic efficacy and can activate some mechanisms related to the generation of long-term potentiation.
在本研究中，研究了酸性成纤维细胞生长因子（aFGF，0.5 - 2.5 ng/ml）对大鼠海马脑片突触传递的影响。刺激施加于Schaffer侧支/连合传入纤维，并在CA1锥体细胞层记录诱发的锋电位。用aFGF持续灌流脑片会轻微降低锋电位的基础幅度，并显著增加双脉冲易化。当在aFGF灌流30分钟后施加短暂强直刺激（100 Hz，7个脉冲）时，经aFGF处理的脑片在强直刺激后增强了短期突触增强的幅度，并促进了长期突触增强的产生。aFGF在强直刺激后也直接增强了强直后增强。aFGF的这些作用具有剂量依赖性。当在强直刺激时或强直刺激后10分钟应用aFGF时，短期突触增强的增强和长期突触增强产生的促进作用不明显。结果表明，aFGF参与突触效能的调节，并可激活一些与长期突触增强产生相关的机制。
