Mancini F P, Newland D L, Mooser V, Murata J, Marcovina S, Young S G, Hammer R E, Sanan D A, Hobbs H H
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-9046, USA.
Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):1911-6. doi: 10.1161/01.atv.15.11.1911.
Transgenic mice expressing transgenes for both human apolipoprotein B-100 (h-apoB) and apolipoprotein(a) [apo(a)] were fed a high-fat, atherogenic diet for 14 weeks to examine the effect of lipoprotein(a) [Lp(a)]on the development of aortic fatty lesions. The extent of lesions in the proximal region of the aorta of Lp(a) mice was measured by use of a computer-assisted image analysis of 20 sections per animal and compared with that of nontransgenic mice as well as mice expressing either the apo(a) or h-apoB transgene. The control (n = 23) and apo(a) (n = 22) transgenic mice had very small mean lesions areas (607 versus 128 microns2 per section). The h-apoB-expressing mice (n = 20) had significantly higher mean lesion areas (3288 microns2 per section) than either the control or apo(a) transgenic animals. Coexpression of apo(a) and h-apoB transgenes resulted in only a modest increase in lesion area (4678 microns2 per section, n = 19). Thus, the expression of human apo(a) in C57BL/6/SJL hybrid mice fed an atherogenic diet failed to significantly potentiate the development of aortic fatty lesions in the absence or presence of high levels of h-apoB.
给同时表达人类载脂蛋白B - 100（h - apoB）和载脂蛋白(a) [apo(a)]转基因的小鼠喂食高脂致动脉粥样化饮食14周，以研究脂蛋白(a) [Lp(a)]对主动脉脂肪病变发展的影响。通过对每只动物的20个切片进行计算机辅助图像分析，测量Lp(a)小鼠主动脉近端区域的病变程度，并与非转基因小鼠以及表达apo(a)或h - apoB转基因的小鼠进行比较。对照（n = 23）和apo(a)（n = 22）转基因小鼠的平均病变面积非常小（每切片分别为607和128平方微米）。表达h - apoB的小鼠（n = 20）的平均病变面积（每切片3288平方微米）显著高于对照或apo(a)转基因动物。apo(a)和h - apoB转基因的共表达仅使病变面积适度增加（每切片4678平方微米，n = 19）。因此，在喂食致动脉粥样化饮食的C57BL/6/SJL杂交小鼠中，无论是否存在高水平的h - apoB，人类apo(a)的表达均未能显著增强主动脉脂肪病变的发展。
