Moreau P, Carosella E, Gluckman E, Gourand L, Prost S, Dausset J, Kirszenbaum M
CEA-DSV, Laboratoire d'immunoradiobiologie, Hôpital Saint-Louis, Centre Hayem, Paris, France.
C R Acad Sci III. 1995 Aug;318(8):837-42.
HLA-G, a non-classical class I gene, is located within the human major histocompatibility complex locus. It has a tissue-specific expression in trophoblast, where the products of HLA-A, -B and -C classical genes are absent. Therefore, the HLA-G gene may have a role during pregnancy in inducing protection of the semi-allogeneic fetus from recognition and destruction by maternal immune cells. The primary transcript of the HLA-G gene is alternatively spliced into 6 mRNA forms (HLA-G1 to HLA-G6), 2 of them may encode soluble forms of the HLA-G antigen (HLA-G5 and HLA-G6). In this work, by using reverse transcription polymerase chain reaction technique, we demonstrate that all transcripts are detected in similar amounts, both in the first and third trimester of gestation. These results are discussed in context of putative function of HLA-G antigen isoforms.
HLA - G是一种非经典的I类基因，位于人类主要组织相容性复合体基因座内。它在滋养层细胞中有组织特异性表达，而经典的HLA - A、- B和- C基因产物在该细胞中不存在。因此，HLA - G基因在孕期可能发挥作用，诱导半同种异体胎儿免受母体免疫细胞的识别和破坏。HLA - G基因的初级转录本可选择性剪接成6种mRNA形式（HLA - G1至HLA - G6），其中2种可能编码HLA - G抗原的可溶性形式（HLA - G5和HLA - G6）。在这项研究中，我们通过逆转录聚合酶链反应技术证明，在妊娠的头三个月和第三个月，所有转录本的检测量相似。我们结合HLA - G抗原异构体的假定功能对这些结果进行了讨论。
