Kobayashi T, Kobayashi E, Sato S, Hotta Y, Miyajima N, Tanaka A, Tabata S
Department of Biology, School of Science, Nagoya University Furoh-cho, Japan.
DNA Res. 1994;1(1):15-26. doi: 10.1093/dnares/1.1.15.
To identify and analyze genes functioning during reproductive cell formation in higher plants, cDNAs harboring the messages induced in meiotic prophase were isolated and characterized. A cDNA library constructed from microsporocytes in meiotic prophase of Lilium longiflorum was screened with a subtraction probe specific to meiotic prophase. Clones selected were classified into 18 groups by cross hybridization and partial sequencing. Northern blot analysis revealed that the transcripts corresponding to the respective cDNA groups began accumulating at the early stages of meiosis and exhibited clone-specific profiles during meiosis and the spore formation process. The amino acid sequences of the predicted gene products showed similarity with known gene products, e.g. heat shock proteins, serine proteases in Bacillus, and RAD 51 gene product in yeast. Half of the putative gene products had hydrophobic N-terminal regions, suggesting that they may function as signal peptides.
为了鉴定和分析高等植物生殖细胞形成过程中发挥作用的基因，分离并鉴定了携带减数分裂前期诱导信息的cDNA。用减数分裂前期特异的扣除探针筛选了从麝香百合减数分裂前期小孢子母细胞构建的cDNA文库。通过交叉杂交和部分测序将所选克隆分为18组。Northern印迹分析表明，与各个cDNA组相对应的转录本在减数分裂早期开始积累，并在减数分裂和孢子形成过程中呈现出克隆特异性图谱。预测的基因产物的氨基酸序列与已知基因产物相似，例如热休克蛋白、芽孢杆菌中的丝氨酸蛋白酶和酵母中的RAD 51基因产物。一半的假定基因产物具有疏水的N端区域，表明它们可能作为信号肽发挥作用。
