Kobayashi T, Hotta Y, Tabata S
Department of Biology, School of Science, Nagoya University, Japan.
Mol Gen Genet. 1993 Feb;237(1-2):225-32. doi: 10.1007/BF00282804.
By using as probe a meiosis-specific cDNA clone LIM15 from the monocotyledonous plant, Lilium longiflorum, a clone containing a 2.8 kb DNA fragment was isolated from a genomic library of Saccharomyces cerevisiae. Primary structure analysis revealed that the clone includes two complete open reading frames, designated ISC2 and ISC10, capable of coding for a 36.6 kDa and a 31.6 kDa polypeptide, respectively, with the former frame being interrupted by a 92 bp intron. The predicted amino acid sequence of Isc2 was 56% identical with the putative gene product of lily cDNA clone LIM15, and showed limited sequence similarity with the yeast RAD57 gene product. Transcripts of the two genes begin accumulating 2.5 h and 7.5 h after induction of meiosis, respectively, according to a Northern hybridization analysis. Since disruption of either one of these genes had a drastic effect on the ability to form spores, ISC2 and ISC10 are expected to play significant roles in the formation of reproductive cells.
通过使用单子叶植物麝香百合的减数分裂特异性cDNA克隆LIM15作为探针，从酿酒酵母的基因组文库中分离出一个包含2.8 kb DNA片段的克隆。一级结构分析表明，该克隆包含两个完整的开放阅读框，分别命名为ISC2和ISC10，它们分别能够编码一个36.6 kDa和一个31.6 kDa的多肽，前一个阅读框被一个92 bp的内含子打断。Isc2的预测氨基酸序列与百合cDNA克隆LIM15的推定基因产物有56%的同一性，并且与酵母RAD57基因产物有有限的序列相似性。根据Northern杂交分析，这两个基因的转录本分别在减数分裂诱导后2.5小时和7.5小时开始积累。由于破坏这两个基因中的任何一个都会对形成孢子的能力产生巨大影响，因此预计ISC2和ISC10在生殖细胞的形成中发挥重要作用。
