编码来自聚球藻属蓝细菌PCC 6301中含I组内含子的tRNA（亮氨酸）和NADH脱氢酶亚基L的基因。

Genes encoding the group I intron-containing tRNA(Leu) and subunit L of NADH dehydrogenase from the cyanobacterium Synechococcus PCC 6301.

作者信息

Sugita M, Luo L, Ohta M, Itadani H, Matsubayashi T, Suguira M

机构信息

Center for Gene Research, Nagoya University, Japan.

出版信息

DNA Res. 1995;2(2):71-6. doi: 10.1093/dnares/2.2.71.

DOI:10.1093/dnares/2.2.71

PMID:7584050

Abstract

A part of the tRNA(Leu)(UAA) gene containing a 240-nucleotide group I intron was amplified by PCR from cyanobacterium Synechococcus PCC 6301 genomic DNA. The pre-tRNA synthesized from the cloned PCR product was efficiently self-spliced in vitro under physiological conditions. The gene encoding the tRNA(Leu)(UAA), trnL-UAA, was isolated from a Synechococcus PCC 6301 genomic library and the nucleotide sequence of a 2,167-bp portion was determined. The trnL-UAA consists of a 34-bp 5' exon, a 240-bp group I intron and a 50-bp 3' exon. In addition, three open reading frames (ORF1, ORF2 and ORF3) were found in the 5' and 3' flanking regions of trnL-UAA. The predicted protein sequence of ORF3, which is located 74-bp upstream from trnL-UAA on the opposite strand, shows 66.2% amino acid identity to that of the Synechocystis PCC 6803 gene encoding subunit L of NADH dehydrogenase (ndhL).

摘要

从蓝藻聚球藻PCC 6301基因组DNA中通过PCR扩增出包含一个240个核苷酸的I组内含子的tRNA(Leu)(UAA)基因的一部分。从克隆的PCR产物合成的前体tRNA在生理条件下能在体外高效地自我剪接。从聚球藻PCC 6301基因组文库中分离出编码tRNA(Leu)(UAA)的基因trnL-UAA，并测定了一个2167 bp片段的核苷酸序列。trnL-UAA由一个34 bp的5'外显子、一个240 bp的I组内含子和一个50 bp的3'外显子组成。此外，在trnL-UAA的5'和3'侧翼区域发现了三个开放阅读框（ORF1、ORF2和ORF3）。位于trnL-UAA上游74 bp处相反链上的ORF3的预测蛋白质序列与编码NADH脱氢酶亚基L（ndhL）的聚球藻PCC 6803基因的蛋白质序列显示出66.2%的氨基酸同一性。