Tekwani B L, Mishra M, Chatterjee R K
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Int J Biochem Cell Biol. 1995 Aug;27(8):851-5. doi: 10.1016/1357-2725(95)00041-m.
Helminth parasites lack the capacity to synthesize polyamines de novo. With the view to characterise alternative pools for the replenishment of polyamines, uptake of spermidine and spermine, were studied in Acanthocheilonema viteae, a parasite known to infect rodents. Motile worms recovered from the subcutaneous tissues of experimentally infected Mastomys natalensis were incubated in vitro with radiolabelled polyamines for the uptake assays. A time-dependent, temperature-sensitive, energy-requiring and saturable-uptake of the polyamines was observed. Male worms exhibited better uptake than the females and spermidine influx occurred at a higher rate than for spermine. A marginal competition of spermine with spermidine uptake and vice-versa was noticed while putrescine did not compete with uptake of either polyamines. Methyl-glyoxal-bis-guanyl hydrazone and Berenil caused significant inhibition of spermidine as well as spermine uptake. Subcutaneous tissues of A. viteae infected animals exhibited markedly higher levels of polyamines compared with the tissues obtained from healthy animals. Filarial worms are thus equipped with multiple polyamine transport systems which may aid their growth and survival within the host.
蠕虫寄生虫缺乏从头合成多胺的能力。为了确定补充多胺的替代来源，对已知感染啮齿动物的寄生虫棘唇旋尾线虫摄取亚精胺和精胺的情况进行了研究。从实验感染的南非乳鼠皮下组织中回收的活动蠕虫，在体外与放射性标记的多胺一起孵育以进行摄取试验。观察到多胺的摄取具有时间依赖性、温度敏感性、能量依赖性和饱和性。雄虫的摄取能力比雌虫更好，亚精胺的流入速率高于精胺。注意到精胺与亚精胺摄取之间存在轻微竞争，反之亦然，而腐胺不与任何一种多胺的摄取竞争。甲基乙二醛双胍腙和贝尼尔显著抑制亚精胺和精胺的摄取。与从健康动物获得的组织相比，感染棘唇旋尾线虫动物的皮下组织中多胺水平明显更高。因此，丝虫配备了多种多胺转运系统，这可能有助于它们在宿主体内的生长和存活。
