Singh R P, Saxena J K, Ghatak S, Shukla O P, Wittich R M, Walter R D
Biochemistry Division, Central Drug Research Institute, Chattar Manzil, Lucknow, India.
Parasitol Res. 1989;75(4):311-5. doi: 10.1007/BF00931816.
Spermine and spermidine were found to be the principal polyamines in the bovine filarial parasite Setaria cervi, whereas putrescine was observed in very low amounts. Studies conducted on the enzymes of polyamine biosynthesis revealed low activity for S-adenosyl-methionine decarboxylase, questionable and negligible activities for the decarboxylation of ornithine and arginine, and appreciable activity for ornithine aminotransferase. Uptake studies with radiolabeled putrescine, spermidine and spermine showed that these amines are rapidly taken up from the medium by an active uptake process. The uptake was temperature-sensitive and abolished at 0-4 degrees C. The questionable presence of biosynthetic enzymes such as ornithine and arginine decarboxylase and, on the other hand, an effective uptake mechanism indicate that the parasite may depend on the host for its polyamine requirement, thereby indicating a possible target for chemotherapy.
精胺和亚精胺被发现是牛丝状寄生虫鹿丝状线虫中的主要多胺，而腐胺的含量极低。对多胺生物合成酶的研究表明，S-腺苷甲硫氨酸脱羧酶活性较低，鸟氨酸和精氨酸脱羧反应的活性存疑且可忽略不计，而鸟氨酸转氨酶活性显著。用放射性标记的腐胺、亚精胺和精胺进行的摄取研究表明，这些胺类通过主动摄取过程迅速从培养基中被摄取。摄取对温度敏感，在0-4摄氏度时会被消除。鸟氨酸和精氨酸脱羧酶等生物合成酶的存在存疑，而另一方面，有效的摄取机制表明该寄生虫的多胺需求可能依赖于宿主，从而提示了一个可能的化疗靶点。
