从片剂包衣制剂中提取和薄层色谱分离食品、药物和化妆品染料。

Extraction and TLC separation of food, drug, and cosmetic dyes from tablet-coating formulations.

作者信息

Fadil F A, McSharry W O

出版信息

J Pharm Sci. 1979 Jan;68(1):97-8. doi: 10.1002/jps.2600680131.

Abstract

A rapid method for extraction of dyes from tablet-coating formulations is described. The dyes were released from their lakes by treatment with concentrated phosphoric acid and dissolved in methanol. After being made alkaline with ammonium hydroxide, the mixture was centrifuged to obtain a clear supernate for application to the TLC plate. With ethyl acetate--methanol--water--concentrated ammonium hydroxide (150:40:35:5) on silica gel, 20 dyes were separated sufficiently to confirm their presence in the coating formulation.

摘要

本文描述了一种从片剂包衣配方中提取染料的快速方法。通过用浓磷酸处理，使染料从其色淀中释放出来并溶解于甲醇中。用氢氧化铵碱化后，将混合物离心以获得澄清的上清液，用于点样于薄层色谱板上。在硅胶板上以乙酸乙酯 - 甲醇 - 水 - 浓氢氧化铵（150:40:35:5）为展开剂，可充分分离出20种染料，从而证实它们存在于包衣配方中。