Prp24与RNA结合的特异性：Prp24在U4和U6小核RNA动态相互作用中的作用
Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs.
作者信息
Ghetti A, Company M, Abelson J
机构信息
Division of Biology 147-75, California Institute of Technology, Pasadena 91125, USA.
出版信息
RNA. 1995 Apr;1(2):132-45.
Prp24 was previously isolated as a suppressor of a cold-sensitive U4 mutation and is required for at least the first step of splicing in vitro. Our investigation of the in vitro RNA binding properties of the purified Prp24 protein shows that it binds preferentially to the U4/U6 hybrid snRNAs compared to other snRNAs. The interaction between Prp24 and the U4/U6 hybrid appears to involve two regions in the RNA: the 39-57 region of U6 and stem II of the U4/U6 hybrid. Interestingly, some U4 mutations, which destabilize stem II, increase the affinity of Prp24 for the U4/U6 RNAs compared to the wild type. This suggests that the binding of Prp24 to the U4/U6 RNAs may involve some destabilization of the RNA duplex. We also found that Prp24 can stimulate the annealing of U4 and U6, suggesting that Prp24 participates in both the formation and disassembly of the U4/U6 hybrid during splicing.
Prp24 先前作为一种冷敏感 U4 突变的抑制因子被分离出来，并且在体外剪接的至少第一步中是必需的。我们对纯化的 Prp24 蛋白的体外 RNA 结合特性的研究表明，与其他 snRNA 相比，它优先结合 U4/U6 杂交 snRNA。Prp24 与 U4/U6 杂交体之间的相互作用似乎涉及 RNA 中的两个区域：U6 的 39 - 57 区域和 U4/U6 杂交体的茎 II。有趣的是，一些使茎 II 不稳定的 U4 突变，与野生型相比，增加了 Prp24 对 U4/U6 RNA 的亲和力。这表明 Prp24 与 U4/U6 RNA 的结合可能涉及 RNA 双链体的一些不稳定。我们还发现 Prp24 可以刺激 U4 和 U6 的退火，这表明 Prp24 在剪接过程中参与了 U4/U6 杂交体的形成和解聚。
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