Shah U, Dasgupta A, Das K M
Department of Medicine, Jersey City Medical Center, New Jersey 07304, USA.
Cell Immunol. 1995 Nov;166(1):154-7. doi: 10.1006/cimm.1995.0017.
Earlier studies have established reactivity of ulcerative colitis colon-bound IgG antibody with an autoantigen recognized by a novel monoclonal antibody, 7E12H12 (IgM isotype). Purified 7E12H12-IgM was used to raise anti-idiotype antibody in the rabbit, and the presence of disease-specific idiotype(s) was evaluated in patients with ulcerative colitis. F(ab')2 fragments were prepared from serum-IgG of the rabbit immunized with 7E12H12-IgM and extensively absorbed against unrelated mouse IgM and purified serum IgG from 4 normal subjects. The reactivity of the absorbed F(ab')2 fragment was tested in an ELISA against the serum from 19 patients with ulcerative colitis, 15 patients with Crohn's disease, and 10 normal subjects. The OD values with ulcerative colitis sera were significantly (P < 0.0005) higher than those with Crohn's disease and normal sera. Blood serum from 84% of patients with ulcerative colitis contains antibodies that are reactive to the F(ab')2 fragments of the immune rabbit serum-IgG. Further studies of the anti-idiotype antibodies may elucidate autoimmune processes in ulcerative colitis and may have diagnostic and therapeutic values.
早期研究已证实溃疡性结肠炎结肠结合IgG抗体与一种新型单克隆抗体7E12H12（IgM同种型）识别的自身抗原具有反应性。用纯化的7E12H12-IgM在兔体内制备抗独特型抗体，并评估溃疡性结肠炎患者中疾病特异性独特型的存在情况。从用7E12H12-IgM免疫的兔血清IgG中制备F(ab')2片段，并与无关小鼠IgM和4名正常受试者的纯化血清IgG进行广泛吸收。用ELISA检测吸收后的F(ab')2片段与19例溃疡性结肠炎患者、15例克罗恩病患者和10名正常受试者血清的反应性。溃疡性结肠炎血清的OD值显著高于（P < 0.0005）克罗恩病血清和正常血清。84%的溃疡性结肠炎患者血清中含有与免疫兔血清IgG的F(ab')2片段反应的抗体。对抗独特型抗体的进一步研究可能阐明溃疡性结肠炎中的自身免疫过程，并可能具有诊断和治疗价值。
