Kicman A T, Coutts S B, Walker C J, Cowan D A
Drug Control Centre, King's College London, UK.
Clin Chem. 1995 Nov;41(11):1617-27.
Currently, there is no recommended confirmatory procedure for detecting doping with the anabolic steroid 5 alpha-dihydrotestosterone (DHT) in sportsmen. To develop a method, we determined ratios of hormone concentrations in urine from 120 healthy men and used these to set discrimination limits. These limits were then applied to results for urine specimens from 10 volunteers given DHT percutaneously (125 mg twice daily for 4 days). The ratio of DHT to epitestosterone (EpiT) was chosen as the primary marker of DHT administration, and ratios of 5 alpha-androstane-3 alpha, 17 beta-diol (5 alpha-ADIOL) to EpiT, 5 alpha-ADIOL to luteinizing hormone, and 5 alpha-ADIOL to 5 beta-androstane-3 alpha, 17 beta-diol were proposed as secondary markers. To evaluate method suitability, we analyzed 194 samples from sports competitors; results for 193 samples were negative, but the ratios in 1 sample greatly exceeded all the chosen limits. In conclusion, we propose that a test scheme based on our recommendations be considered for implementation in all Olympic drug-testing laboratories.
目前，对于检测运动员是否使用合成代谢类固醇5α - 双氢睾酮（DHT）进行兴奋剂 doping，尚无推荐的确认程序。为开发一种方法，我们测定了120名健康男性尿液中的激素浓度比值，并以此设定判别限。然后将这些限值应用于10名志愿者经皮给予DHT（每天两次，每次125mg，共4天）后的尿液样本检测结果。选择DHT与表睾酮（EpiT）的比值作为DHT使用的主要标志物，并提出5α - 雄烷 - 3α，17β - 二醇（5α - ADIOL）与EpiT的比值、5α - ADIOL与促黄体生成素的比值以及5α - ADIOL与5β - 雄烷 - 3α，17β - 二醇的比值作为次要标志物。为评估方法的适用性，我们分析了194份来自体育竞赛选手的样本；193份样本结果为阴性，但有1份样本的比值大大超过了所有选定的限值。总之，我们建议考虑在所有奥林匹克药物检测实验室实施基于我们建议的检测方案。
