Delemarre F G, Stevenhagen A, Van Furth R
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
Clin Exp Immunol. 1995 Nov;102(2):425-9. doi: 10.1111/j.1365-2249.1995.tb03800.x.
In this study we investigated the effect of human GM-CSF on the toxoplasmastatic activity and release of H2O2 and PGE2 by human monocytes. Incubation of monocytes from healthy controls with GM-CSF resulted in a dose-dependent reduction of toxoplasmastatic activity and a decrease in H2O2 production. Furthermore GM-CSF-treated monocytes released more PGE2 than untreated cells. To investigate the role of PGE2 in the reduced toxoplasmastatic activity of GM-CSF-treated monocytes, these cells were incubated with indomethacin. This resulted in a reduction of PGE2 release and restoration of toxoplasmastatic activity of monocytes treated with GM-CSF. GM-CSF reduces the toxoplasmastatic activity of monocytes via production of PGE2.
在本研究中，我们调查了人粒细胞-巨噬细胞集落刺激因子（GM-CSF）对人单核细胞的抗弓形虫活性以及过氧化氢（H2O2）和前列腺素E2（PGE2）释放的影响。将健康对照者的单核细胞与GM-CSF一起孵育，导致抗弓形虫活性呈剂量依赖性降低，且H2O2生成减少。此外，经GM-CSF处理的单核细胞比未处理的细胞释放更多的PGE2。为了研究PGE2在GM-CSF处理的单核细胞抗弓形虫活性降低中的作用，将这些细胞与吲哚美辛一起孵育。这导致PGE2释放减少，且GM-CSF处理的单核细胞的抗弓形虫活性得以恢复。GM-CSF通过PGE2的产生降低单核细胞的抗弓形虫活性。
