Thomassen M J, Antal J M, Divis L T, Wiedemann H P
Department of Pulmonary, Cleveland Clinic Foundation, Ohio 44195-5038, USA.
Clin Immunol Immunopathol. 1995 Nov;77(2):201-5. doi: 10.1006/clin.1995.1144.
We previously demonstrated that the synthetic surfactant Exosurf and a modified natural surfactant, Survanta, both down-regulated endotoxin-stimulated production of inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6) in human alveolar macrophages. To further characterize the source of the inhibitory effect of surfactant, the three individual components of Exosurf were evaluated. Dipalmitoylphosphatidylcholine had no effect on endotoxin-stimulated cytokine secretion. Cetyl alcohol (spreading agent) compromised macrophage function as measured by adherence. However, at concentrations equivalent to those found in the complete surfactant (Exosurf) preparation, tyloxapol (nonionic dispersing agent) was inhibitory in a dose-dependent manner. The viability of alveolar macrophages as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide cleavage assay was not affected by incubation in Exosurf or any of its individual components. Cytokine secretion and mRNA levels of endotoxin-stimulated alveolar macrophages were decreased by tyloxapol. These data suggest that tyloxapol alone, like Exosurf, has an inhibitory effect on cytokine production which may be pretranslationally mediated.
我们之前证明，合成表面活性剂Exosurf和一种改良的天然表面活性剂Survanta，均可下调人肺泡巨噬细胞中内毒素刺激的炎性细胞因子（肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6）的产生。为了进一步明确表面活性剂抑制作用的来源，我们对Exosurf的三种单独成分进行了评估。二棕榈酰磷脂酰胆碱对内毒素刺激的细胞因子分泌没有影响。十六醇（铺展剂）通过黏附作用检测发现损害了巨噬细胞功能。然而，在与完整表面活性剂（Exosurf）制剂中浓度相当的情况下，聚氧乙烯辛基苯基醚（非离子分散剂）呈剂量依赖性抑制作用。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑裂解试验评估，肺泡巨噬细胞的活力不受Exosurf或其任何单独成分孵育的影响。聚氧乙烯辛基苯基醚可降低内毒素刺激的肺泡巨噬细胞的细胞因子分泌和mRNA水平。这些数据表明，单独的聚氧乙烯辛基苯基醚与Exosurf一样，对细胞因子产生具有抑制作用，这可能是在翻译前介导的。
