Koch A E, Kunkel S L, Shah M R, Fu R, Mazarakis D D, Haines G K, Burdick M D, Pope R M, Strieter R M
Departments of Medicine, Northwestern University Medical School, Chicago, Illinois, USA.
Clin Immunol Immunopathol. 1995 Dec;77(3):307-14. doi: 10.1006/clin.1995.1157.
The aim of this study was to determine whether the cytokine macrophage inflammatory protein-1 beta (MIP-1 beta) is present and functionally active in the arthritic joint. We used immunoassays and bioassays to assess the presence and function of MIP-1 beta using samples obtained from 62 arthritic patients. MIP-1 beta levels were increased in synovial fluids (SFs) from patients with osteoarthritis (OA) (18.0 +/- 8.9 ng/ml) (SD) compared to patients with rheumatoid arthritis (RA) 6.1 +/- 2.9 ng/ml) or other forms of arthritis (10.4 +/- 7.0 ng/ml) (P < 0.05). Levels of OA SF MIP-1 beta were significantly greater than OA or normal serum levels of MIP-1 beta. Anti-MIP-1 beta neutralized 28% of the chemotactic activity for monocytes found in OA SFs. Isolated OA synovial tissue fibroblasts did not constitutively produce MIP-1 beta but could be induced to express this chemokine upon exposure to tumor necrosis factor-alpha, interleukin-1 beta, or lipopolysaccharide. Synovial tissue immunohistochemical staining revealed that the main immunopositive cells in OA were the lining cells as well as vascular smooth muscle and endothelial cells. A minority of macrophages were immunopositive as well. In this study, we identify MIP-1 beta as a unique cytokine increased in OA compared to RA SF. We conclude that MIP-1 beta may play a role in the ingress of monocytes into the OA joint.
本研究的目的是确定细胞因子巨噬细胞炎性蛋白-1β（MIP-1β）在关节炎关节中是否存在并具有功能活性。我们使用免疫测定法和生物测定法，通过从62例关节炎患者获取的样本评估MIP-1β的存在和功能。与类风湿性关节炎（RA）患者（6.1±2.9 ng/ml）或其他形式关节炎患者（10.4±7.0 ng/ml）相比，骨关节炎（OA）患者滑液（SF）中的MIP-1β水平升高（18.0±8.9 ng/ml）（标准差）（P<0.05）。OA滑液MIP-1β水平显著高于OA或正常血清MIP-1β水平。抗MIP-1β中和了OA滑液中发现的单核细胞趋化活性的28%。分离出的OA滑膜组织成纤维细胞不组成性产生MIP-1β，但在暴露于肿瘤坏死因子-α、白细胞介素-1β或脂多糖后可被诱导表达这种趋化因子。滑膜组织免疫组化染色显示，OA中主要的免疫阳性细胞是衬里细胞以及血管平滑肌和内皮细胞。少数巨噬细胞也呈免疫阳性。在本研究中，我们确定与RA滑液相比，MIP-1β是OA中升高的一种独特细胞因子。我们得出结论，MIP-1β可能在单核细胞进入OA关节过程中起作用。
