Courtoy R, Boniver J, Simar L
Histochemistry. 1978 Mar 2;55(2):107-15. doi: 10.1007/BF00493513.
Thin sections in mouse mast cells and thymic cells are stained with cobalt thiocyanate a compound known to form insoluble complexes with organic bases. Chromatin, nucleolus, ribosomes and mast cell granules are contrasted. Different blockade reactions and enzymatic digestions indicate the staining corresponds to the basic protein amino-groups. The silver methenamine reaction stains the same cellular structures. However, the specificity control reactions show the staining mainly corresponds to protein sulphydryle groups and in a lesser extent to aldehyde and polyanions.
小鼠肥大细胞和胸腺细胞的薄切片用硫氰酸钴染色，硫氰酸钴是一种已知能与有机碱形成不溶性复合物的化合物。染色质、核仁、核糖体和肥大细胞颗粒呈现出对比。不同的封闭反应和酶消化表明，这种染色对应于碱性蛋白质的氨基。六胺银反应可对相同的细胞结构进行染色。然而，特异性对照反应表明，这种染色主要对应于蛋白质的巯基，在较小程度上对应于醛和多阴离子。
