Joyce N C, Joyce S J, Powell S M, Meklir B
Schepens Eye Research Institute, Boston, MA 02114, USA.
Curr Eye Res. 1995 Jul;14(7):601-9. doi: 10.3109/02713689508998408.
In vivo repair of the adult human corneal endothelium occurs mainly by movement of cells into the wound defect rather than by cell division. Two forms of cell movement contribute to endothelial wound repair: migration of individual cells into the defect and spreading of the confluent monolayer into the wound area. This laboratory has developed a tissue culture model using rabbit corneal endothelial cells pretreated with the mitotic inhibitor 5-fluorouracil to mimic the relatively amitotic state of human corneal endothelium in vivo. This model permits study of the effects of growth factors and other agents on individual cell migration and monolayer spreading in response to wounding. mRNA for epidermal growth factor (EGF) and its receptor has been detected in cultured corneal endothelial cells and EGF receptors have been detected on human corneal endothelial cells in situ, suggesting that this growth factor may act in an autocrine manner. Prostaglandin E2 (PGE2) is synthesized by cultured corneal endothelial cells and is present in relatively high quantity in aqueous humor in response to corneal wounding and to inflammation in the anterior chamber. Although corneal endothelial cells may be exposed to both EGF and PGE2, little is known about their effects on monolayer repair. The current study compared the effects of PGE2 alone, EGF alone, and both agents in combination on individual cell migration and monolayer spreading using the wound model system and also determined the effect of EGF on PGE2 secretion using a commercial immunoassay. A 15 min exposure of wounded cultures to exogenous PGE2 stimulated individual cell migration and suppressed monolayer spreading.(ABSTRACT TRUNCATED AT 250 WORDS)
成体人类角膜内皮细胞的体内修复主要通过细胞向伤口缺损处移动来实现，而非细胞分裂。细胞移动的两种形式有助于内皮伤口修复：单个细胞迁移至缺损处以及融合的单层细胞向伤口区域扩展。本实验室已开发出一种组织培养模型，使用经有丝分裂抑制剂5-氟尿嘧啶预处理的兔角膜内皮细胞，以模拟人类角膜内皮细胞在体内相对无丝分裂的状态。该模型可用于研究生长因子和其他试剂对单个细胞迁移以及单层细胞对伤口做出反应时扩展的影响。在培养的角膜内皮细胞中已检测到表皮生长因子（EGF）及其受体的mRNA，并且在人角膜内皮细胞原位也检测到了EGF受体，这表明该生长因子可能以自分泌方式起作用。前列腺素E2（PGE2）由培养的角膜内皮细胞合成，并且在角膜受伤和前房炎症反应时，房水中的含量相对较高。尽管角膜内皮细胞可能同时接触EGF和PGE2，但它们对单层修复的影响却知之甚少。本研究使用伤口模型系统比较了单独的PGE2、单独的EGF以及两者联合使用对单个细胞迁移和单层扩展的影响，并且还使用商业免疫测定法确定了EGF对PGE2分泌的影响。将受伤的培养物暴露于外源性PGE2 15分钟可刺激单个细胞迁移并抑制单层扩展。（摘要截短于250字）
