Nakahori Y, Katakami C
Department of Ophthalmology, Kobe University School of Medicine, Hyogo-ken, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Jan;99(1):17-22.
We investigated the effect of epidermal growth factor (EGF) on rabbit corneal endothelial wound healing in vivo. After a 6 mm-diameter metallic cryoprobe was applied to rabbit corneas, 0.1 ml of recombinant human EGF (100 micrograms/ml) or saline was injected into the anterior chamber. Corneas were excised on 1, 2, and 7 days postoperatively, labeled with 3H-thymidine and subjected to autoradiography. Some corneas were examined by scanning electron microscopy. Autoradiography showed that the number of endothelial cells incorporating 3H-thymidine in corneas treated with EGF was significantly greater than that in the control. Scanning electron microscopy demonstrated the effect of EGF on accelerating endothelial healing. These findings indicate that EGF has a stimulatory effect on the proliferation of wounded rabbit corneal endothelial cells in vivo. Under the conditions tested in the present study, there were no side effects of EGF such as neovascularization or cellular proliferation in the angle. The results suggest that EGF might be clinically applicable for corneal endothelial disorders.
我们研究了表皮生长因子（EGF）对兔角膜内皮细胞体内伤口愈合的影响。使用直径6毫米的金属冷冻探头作用于兔角膜后，将0.1毫升重组人表皮生长因子（100微克/毫升）或生理盐水注入前房。术后第1、2和7天切除角膜，用3H-胸腺嘧啶核苷标记并进行放射自显影。部分角膜通过扫描电子显微镜检查。放射自显影显示，用表皮生长因子处理的角膜中摄取3H-胸腺嘧啶核苷的内皮细胞数量显著多于对照组。扫描电子显微镜证实了表皮生长因子对加速内皮愈合的作用。这些发现表明，表皮生长因子对兔角膜内皮细胞体内伤口的增殖具有刺激作用。在本研究测试的条件下，表皮生长因子没有诸如新生血管形成或房角细胞增殖等副作用。结果表明，表皮生长因子可能在临床上适用于角膜内皮疾病。
