Tiirikainen M I
Blood Transfusion Service, Finnish Red Cross, Helsinki.
Cytometry. 1995 Aug 1;20(4):341-8. doi: 10.1002/cyto.990200410.
When analyzing leukocyte cell surface antigens by flow cytometry, leukocytes are usually first labeled in whole blood and the red blood cells are finally lysed with lysing solutions. The erythrocytes are lysed, but the leukocytes are expected to remain intact. Six commercial red blood cell lysing methods were investigated for possible leukocyte permeabilization effect. The effectiveness of permeabilization was studied by propidium iodide staining, and the detectability of intracellular antigens was studied by using monoclonal antibodies toward two model antigens. Most of the lysing methods caused permeabilization of at least part of the leukocytes, but only one method, already found in our previous studies, was applicable for complete permeabilization of leukocytes and for detection of intracellular antigens alone or simultaneously with the cell surface antigens.
通过流式细胞术分析白细胞细胞表面抗原时，通常先在全血中标记白细胞，最后用裂解液裂解红细胞。红细胞被裂解，但白细胞应保持完整。研究了六种商用红细胞裂解方法可能产生的白细胞通透化效应。通过碘化丙啶染色研究通透化的有效性，并用针对两种模型抗原的单克隆抗体研究细胞内抗原的可检测性。大多数裂解方法至少会使部分白细胞发生通透化，但只有一种方法（我们之前的研究中已发现）适用于白细胞的完全通透化以及单独或与细胞表面抗原同时检测细胞内抗原。
