Lucia B, Jennings C, Cauda R, Ortona L, Landay A L
Instituto di Clinica delle Malattie Infettive Universita Cattolica S. Cuore Largo A. Gemelli, Roma, Italy.
Cytometry. 1995 Mar 15;22(1):10-5. doi: 10.1002/cyto.990220103.
Three-color flow cytometric analysis of CD16+ natural killer (NK) cells was assessed in HIV seropositive patients and healthy heterosexual controls. A selective depletion of lymphocytes with the CD16+ NK phenotype was found among the HIV+ infected patients. When the CD16 lymphocyte subset was further evaluated by three-color flow cytometry, cells bearing both the CD8 and CD56 antigens were significantly decreased. Analysis of activation antigens revealed a large proportion of CD16+ NK cells from HIV+ patients expressed HLA-DR, but this did not correlate with CD25 (IL-2 receptor) expression. The overall loss of the CD8 and CD56 antigens among the NK population with an increase in activation status may be due to differential loss of the NK cell subsets or, alternatively, to the loss of immunoregulatory cytokines, which have been shown to be important in maintaining NK activity. Whether these changes in the NK compartment may influence the outcome of individuals with HIV disease still remains an open question but is an important issue when performing phenotypic analysis of HIV+ subjects.
在HIV血清阳性患者和健康异性对照中评估了CD16 +自然杀伤（NK）细胞的三色流式细胞术分析。在HIV +感染患者中发现具有CD16 + NK表型的淋巴细胞选择性耗竭。当通过三色流式细胞术进一步评估CD16淋巴细胞亚群时，同时携带CD8和CD56抗原的细胞显著减少。激活抗原分析显示，来自HIV +患者的大部分CD16 + NK细胞表达HLA-DR，但这与CD25（IL-2受体）表达无关。NK群体中CD8和CD56抗原的总体丧失以及激活状态的增加可能是由于NK细胞亚群的差异性丧失，或者是由于免疫调节细胞因子的丧失，而免疫调节细胞因子已被证明在维持NK活性中很重要。NK区室的这些变化是否会影响HIV疾病个体的预后仍然是一个悬而未决的问题，但在对HIV +受试者进行表型分析时是一个重要问题。
