Seamer L C, Altobelli K K
University of New Mexico, Albuquerque, USA.
Cytometry. 1995 Mar 15;22(1):60-4. doi: 10.1002/cyto.990220111.
The goal of this work was to develop an objective, quantitative, and reproducible method of detecting fluorescence drift which may have occurred during DNA cell-cycle data acquisition. Quality control software, "TruPloid," is described that analyzes list-mode files to detect and quantify fluorescence drift using three separate statistical tests. We show that fluorescence drift may lead to a variety of measurement artifacts including high coefficients of variation, obscuring of small populations and creation of distinct artificial peaks. Forty percent of 50 archived list-mode files displayed fluorescence drift, which demonstrates the need for detection methods to deal with this source of DNA cell-cycle histogram artifacts.
这项工作的目标是开发一种客观、定量且可重复的方法，用于检测在DNA细胞周期数据采集过程中可能发生的荧光漂移。文中描述了质量控制软件“TruPloid”，它通过三种独立的统计测试来分析列表模式文件，以检测和量化荧光漂移。我们表明，荧光漂移可能导致各种测量伪像，包括高变异系数、小群体的模糊以及明显人工峰的产生。50个存档的列表模式文件中有40%显示出荧光漂移，这表明需要检测方法来处理这种DNA细胞周期直方图伪像的来源。
