Watson J V
MRC Clinical Oncology Unit, Medical School, Cambridge, England.
Cytometry. 1987 Nov;8(6):646-9. doi: 10.1002/cyto.990080619.
Time has been used as a quality-control parameter in our flow cytometer. This parameter was automatically incorporated into the list-mode data base by hooking the computer clock directly into the acquisition logic. Quality control can then be checked by "replaying" fluorescence or light scatter data vs. time and any instrumental drift can be observed and degraded data can be excised. The technique is illustrated with a number of DNA data sets from a tissue culture cell line in which artificial perturbations were introduced during data acquisition to simulate potential causes of instrumental drift.
时间已被用作我们流式细胞仪的质量控制参数。通过将计算机时钟直接连接到采集逻辑中，该参数被自动纳入列表模式数据库。然后可以通过“重放”荧光或光散射数据与时间的关系来检查质量控制，并且可以观察到任何仪器漂移，还可以剔除质量下降的数据。文中用来自一个组织培养细胞系的多个DNA数据集对该技术进行了说明，在数据采集过程中引入了人工干扰，以模拟仪器漂移的潜在原因。
