Bashaw G J, Baker B S
Department of Biological Sciences, Stanford University, CA 94305, USA.
Development. 1995 Oct;121(10):3245-58. doi: 10.1242/dev.121.10.3245.
In Drosophila dosage compensation increases the rate of transcription of the male's X chromosome and depends on four autosomal male-specific lethal genes. We have cloned the msl-2 gene and shown that MSL-2 protein is co-localized with the other three MSL proteins at hundreds of sites along the male polytene X chromosome and that this binding requires the other three MSL proteins. msl-2 encodes a protein with a putative DNA-binding domain: the RING finger. MSL-2 protein is not produced in females and sequences in both the 5' and 3' UTRs are important for this sex-specific regulation. Furthermore, msl-2 pre-mRNA is alternatively spliced in a Sex-lethal-dependent fashion in its 5' UTR.
在果蝇中，剂量补偿提高了雄性X染色体的转录速率，并且依赖于四个常染色体雄性特异性致死基因。我们已经克隆了msl-2基因，并表明MSL-2蛋白与其他三种MSL蛋白在雄性多线X染色体上的数百个位点共定位，并且这种结合需要其他三种MSL蛋白。msl-2编码一种具有推定DNA结合结构域（即环指结构域）的蛋白质。雌性不产生MSL-2蛋白，5'和3'非翻译区的序列对于这种性别特异性调控很重要。此外，msl-2前体mRNA在其5'非翻译区以性别致死因子依赖的方式进行可变剪接。
